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PE/Cyanine7 anti-human CD261 (DR4, TRAIL-R1) Antibody

Pricing & Availability
Clone
DJR1 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
TRAIL-R1, Apo-2, CD261, TNFRSF10A
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
DJR1_PECyanine7_CD261_Antibody_1_070519
Human DR4 Transfected BHK cell line was stained with CD261 (DR4, TRAIL-R1) (clone DJR1) PE/Cyanine7 (filled histogram) or Mouse IgG1, ? PE/Cyanine7 isotype control (open histogram).
  • DJR1_PECyanine7_CD261_Antibody_1_070519
    Human DR4 Transfected BHK cell line was stained with CD261 (DR4, TRAIL-R1) (clone DJR1) PE/Cyanine7 (filled histogram) or Mouse IgG1, ? PE/Cyanine7 isotype control (open histogram).
Compare all formats See PE/Cyanine7 spectral data
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307209 25 tests £109
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307210 100 tests £254
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Description

DR4 is 56 kD member 10A of the TNFR superfamily (TNFRSF10A), also known as TRAIL-R1, Apo-2, and CD261. It is expressed at low levels by activated T cells and some tumors. After TRAIL engagement, DR4 (TRAIL-R1), through activation of NF-κB, induces apoptosis in the TRAIL ligated cell.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of DR4-human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: The DJR1 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR4/TRAIL-R1 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307206) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Uno K, et al. 2003. Blood 101:3658.
  2. Sato K, et al. 2005. J. Immunol. 174:4025.
  3. Lundqvist A, et al. 2006. Cancer Res. 66:7317. PubMed
  4. Chen KF, et al. 2011. J Pharmacol Exp Ther. 337:155. PubMed
  5. Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed
  6. Chandrasekaran S, et al. 2014. PLoS One. 9:111487. PubMed
RRID
AB_2814152 (BioLegend Cat. No. 307209)
AB_2814153 (BioLegend Cat. No. 307210)

Antigen Details

Structure
TNFR superfamily, 56 kD
Distribution

Activated T cells, tumor cells

Function
Induces apoptosis
Ligand/Receptor
TRAIL
Cell Type
T cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Macfarlane M. 2003. Toxicol. Lett. 139:89.
2. Baetu T, et al. 2002. Cytokine Growth Factor Rev. 13:199.
3. Degli-Esposti M. 1999. J. Leukoc. Biol. 65:535.

Gene ID
8797 View all products for this Gene ID
UniProt
View information about CD261 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/01/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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