PE/Cyanine7 anti-human C3AR Antibody

Pricing & Availability
Clone
hC3aRZ8 (See other available formats)
Other Names
C3a anaphylatoxin chemotactic receptor, C3a-R, AZ3B, HNFAG09
Isotype
Mouse IgG2b
Ave. Rating
Submit a Review
Product Citations
publications
hC3aRZ8_PECyanine7_C3AR_Antibody_1_061719
Human peripheral blood granulocytes were stained with human C3AR (clone hC3aRZ8) PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype contrl (open histogram).
  • hC3aRZ8_PECyanine7_C3AR_Antibody_1_061719
    Human peripheral blood granulocytes were stained with human C3AR (clone hC3aRZ8) PE/Cyanine7 (filled histogram) or mouse IgG2b, κ PE/Cyanine7 isotype contrl (open histogram).
Cat # Size Price Quantity Avail. Save
345807 25 tests £104
Check Availability


Need larger quantities of this item?
Request Bulk Quote
345808 100 tests £218
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

C3AR is a single chain protein of 482 aa, with seven membrane-spanning regions and a molecular weight of 54 Kd. C3AR is expressed by neutrophils, eosinophils, mast cells, monocytes, dendritic cells and there are reports of C3AR expression in non-immune cells such as hematopoietic and mesenchymal stem cells, epithelial and endothelial cells. C3AR is coupled to heterotrimeric G proteins and after C3a binding, the signal transduced, results in chemotaxis, granule enzyme release and superoxide anion production.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
hC3aR transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions. The solution is free of unconjugated PE/Cyanine7 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Soruri A, et al. 2002. J. Immunol. 170:3306 (FC)
  2. Kiafard Z, et al. 2007 Immunobiology. 212:129 (FC, IHC)

Antigen Details

Structure
482 aa, single chain protein with seven membrane-spanning regions, 54 kD
Distribution

Neutrophils, eosinophils, mast cells, monocytes, dendritic cells, hematopoietic and mesenchymal stem cells, endothelial cells

Function
After C3a binding, the C3AR signaling results in chemotaxis, granule enzyme release and superoxide anion production.
Interaction
G proteins
Bioactivity
Chemotaxis, degranulation
Cell Type
Dendritic cells, Endothelial cells, Eosinophils, Mast cells, Mesenchymal Stem Cells, Monocytes, Neutrophils
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Wysoczynski M et al. 2009. Leukemia. 23:1455
2. Schraufstatter IU. et al. 2009. J. Immunol. 182:3827
3. Honczarenko M et al. 2005. J. Immunol. 175:3698
4. Reca R et al. 2003. Blood 101:3784

Gene ID
719 View all products for this Gene ID
UniProt
View information about C3aR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/17/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account