PE/Cy7 anti-human CD309 (VEGFR2) Antibody

Pricing & Availability
A16085H (See other available formats)
Other Names
Mouse IgG1, κ
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HUVEC human endothelial cells were stained with CD309 (clone A16085H) PE/Cy7 (filled histogram) or mouse IgG1, κ isotype control (open histogram).
  • A16085H_PEY7_CD309_Antibody_103018
    HUVEC human endothelial cells were stained with CD309 (clone A16085H) PE/Cy7 (filled histogram) or mouse IgG1, κ isotype control (open histogram).
See PE/Cy7 spectral data
Cat # Size Price Quantity Avail. Save
393007 25 tests £101
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393008 100 tests £218
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CD309, also known as VEGF-R2, KDR, and Flk-1 (mouse), is a type I transmembrane glycoprotein. It is a member of the CSF-1/PDGF receptor family of type III tyrosine kinase receptors. Human VEGF-R2 is mainly expressed by endothelial cells, embryonic tissues, and megakaryocytes. It plays an important role in the regulation of angiogenesis, vasculogenesis, and vascular permeability. The ligands of VEGF-R2 include VEGF-A, VEGF-C, and VEGF-D splice isoforms. Activation of VEGF-R2 with its ligands results in the receptor dimerization and autophosphorylation, stimulating endothelial cell proliferation and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Recombinant Human VEGFR2-Fc Chimera
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone does not cross-react with mouse endothelial cells.

AB_2783296 (BioLegend Cat. No. 393007)
AB_2783297 (BioLegend Cat. No. 393008)

Antigen Details

Type I transmembrane glycoprotein, type III tyrosine kinase receptor, CSF-1/PDGF receptor family

Endothelial cells, embryonic tissues

VEGFR2 regulates angiogenesis as well as vascular development and permeability.
Ligand Receptor
VEGF-A, VEGF-C, and VEGF-D splice isoforms
Cell Type
Endothelial cells
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. DiSalvo J, et al. 1995. J Biol Chem. 270:7717.
  2. McColl BK, et al. 2003. J Exp Med. 198:863.
  3. Albuquerque RJ, et al. 2009. Nat Med. 15:1023.
  4. Guangqi E, et al. 2012. J Biol Chem. 287:3029.
  5. Koch S, and Claesson-Welsh. 2012. Cold Spring Harb Perspect Med. 2:a006502.
  6. Munaut C, et al. 2012. Plos One. 7:e33475.
  7. Wehland M, et al. 2013. Int J Mol Sci. 14:9338.
Gene ID
3791 View all products for this Gene ID
View information about CD309 on

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Go To Top Version: 1    Revision Date: 10/30/2018

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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