PE/Dazzle™ 594 anti-human CD137L (4-1BB Ligand) Antibody

Pricing & Availability
Clone
5F4 (See other available formats)
Other Names
4-1BBL, CDw137L, TNFSF9, 41BBL
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5F4_PEDazzle594_CD137L_Antibody_062918
Human T lymphoblastic leukemia cell line, Hut-78, was stained with CD137L (4-1BB Ligand, clone 5F4) PE/Dazzle™ 594 (filled histogram) or Mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
  • 5F4_PEDazzle594_CD137L_Antibody_062918
    Human T lymphoblastic leukemia cell line, Hut-78, was stained with CD137L (4-1BB Ligand, clone 5F4) PE/Dazzle™ 594 (filled histogram) or Mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Avail. Save
311515 25 tests £91
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311516 100 tests £215
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Description

4-1BB ligand, also known as CDw137L, is a 97 kD member of the TNF superfamily mainly expressed on APCs, activated B and T cells. It has been reported to be important in T cell proliferation and cytokine production through interaction with 4-1BB receptor. 4-1BB ligand appears to be able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 311504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Gullo C, et al. 2010. PLoS One. 5:e10845. (FC) PubMed
RRID
AB_2734285 (BioLegend Cat. No. 311515)
AB_2783180 (BioLegend Cat. No. 311516)

Antigen Details

Structure
TNF superfamily, 97 kD
Distribution

Antigen presenting cells, activated B cells and T cells

Function
T cell proliferation, cytokine production
Ligand Receptor
4-1BB (CDw137)
Cell Type
Antigen-presenting cells, B cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Akiba H, et al. 2000. J. Exp. Med. 191:375. 
2. Pollak KE, et al. 1995. Eur. J. Immunol. 25:488. 
3. DeBenedette MA, et al. 1997. J. Immunol. 158:551. 
4. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.

Gene ID
8744 View all products for this Gene ID
UniProt
View information about CD137L on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/29/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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