PE/Dazzle 594 anti-human CD11b Antibody anti-CD11b

Pricing & Availability

Clone: ICRF44 (See other available formats)
Regulatory Status: RUO
Workshop: IV M047
Other Names: Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood granulocytes were stained with CD11b (clone ICRF44) PE/Dazzle™ 594 (filled histogram) or mouse IgG1, κ PE/Dazzle™ 594 isotype control (open histogram).

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301347

25 tests

£105

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301348

100 tests

£247

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Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as α_M integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β₂ (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Chimpanzee, Common Marmoset, Pig

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy⁵, stimulation of monocytes³, blocking of heterotypic PMN aggregation⁸, and blocking of granulocyte activation¹². This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Application References (PubMed link indicates BioLegend citation)

Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
Rezzonico R, et al. 2001. Blood 97:2932. (Stim)
Marsik C, et al. 2003. Shock 20:493. (FC)
David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF)
Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
Thurlow LR, et al. 2010. Infect. Immun. 128:1128. (FC) PubMed
Jadhav S, et al. 2001. J. Immunol. 167:5986. (Block)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. (FC)
Wen T, et al. 2014. J Immunol. 192:5481. (FC) PubMed
Sprong T, et al. 2003. Blood 102:3702. (Block)
Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
Larsson K, et al. 2015. PNAS. PubMed

Product Citations

Giamarellos-Bourboulis EJ, et al. 2020. Cell. 183(2):315-323.e9. PubMed
Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
Moorlag SJCFM, et al. 2020. Cell Rep. 33:108387. PubMed
Routhu NK, et al. 2021. Immunity. 54(3):542-556.e9. PubMed

RRID

AB_2564080 (BioLegend Cat. No. 301347)
AB_2564081 (BioLegend Cat. No. 301348)

Antigen Details

Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β₂ (CD18), 165-170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells
Function: Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor: ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type: B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.
Gene ID: 3684 View all products for this Gene ID
UniProt: View information about CD11b on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Pathways

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Other Formats

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Alexa Fluor® 488 anti-human CD11b	ICRF44	FC
Alexa Fluor® 647 anti-human CD11b	ICRF44	FC
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PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
Brilliant Violet 421™ anti-human CD11b	ICRF44	FC,ICC
Brilliant Violet 570™ anti-human CD11b	ICRF44	FC
FITC anti-human CD11b	ICRF44	FC
Brilliant Violet 605™ anti-human CD11b	ICRF44	FC
Brilliant Violet 510™ anti-human CD11b	ICRF44	FC,ICC
Brilliant Violet 650™ anti-human CD11b	ICRF44	FC
Purified anti-human CD11b (Maxpar® Ready)	ICRF44	FC,CyTOF®
Alexa Fluor® 594 anti-human CD11b	ICRF44	ICC,FC
APC/Cyanine7 anti-human CD11b	ICRF44	FC
Brilliant Violet 711™ anti-human CD11b	ICRF44	FC
Brilliant Violet 785™ anti-human CD11b	ICRF44	FC
PE/Dazzle™ 594 anti-human CD11b	ICRF44	FC
APC/Fire™ 750 anti-human CD11b	ICRF44	FC
APC anti-human CD11b	ICRF44	FC
PE anti-human CD11b	ICRF44	FC
TotalSeq™-A0161 anti-human CD11b	ICRF44	PG
Alexa Fluor® 700 anti-human CD11b	ICRF44	FC
PE/Cyanine7 anti-human CD11b	ICRF44	FC
TotalSeq™-B0161 anti-human CD11b	ICRF44	PG
TotalSeq™-C0161 anti-human CD11b	ICRF44	PG
PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
Ultra-LEAF™ Purified anti-human CD11b	ICRF44	FC,CyTOF®,Block,ICC,IHC
TotalSeq™-D0161 anti-human CD11b	ICRF44	PG
GMP PE/Cyanine7 anti-human CD11b	ICRF44	FC
Pacific Blue™ anti-human CD11b	ICRF44	FC
GMP PE anti-human CD11b	ICRF44	FC
Spark UV™ 387 anti-human CD11b	ICRF44	FC
GMP PerCP/Cyanine5.5 anti-human CD11b	ICRF44	FC
FITC anti-human CD11b	ICRF44	FC
APC/Fire™ 750 anti-human CD11b	ICRF44	FC
GMP APC anti-human CD11b	ICRF44	FC
GMP FITC anti-human CD11b	ICRF44	FC
GMP APC/Fire™ 750 anti-human CD11b	ICRF44	FC
Spark Blue™ 515 anti-human CD11b	ICRF44	FC
Spark Violet™ 500 anti-human CD11b	ICRF44	FC
Spark Red™ 718 anti-human CD11b (Flexi-Fluor™)	ICRF44	FC

Certificate of Analysis

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Go To Top Version: 4 Revision Date: 07/13/2015

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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