PE/Cyanine7 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_PECy7_083109
C57BL/6 bone marrow cells stained with HK1.4 PE/Cyanine7
  • HK1dot4_PECy7_083109
    C57BL/6 bone marrow cells stained with HK1.4 PE/Cyanine7
See PE/Cyanine7 spectral data
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128017 25 µg £72.00
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128018 100 µg £181.00
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
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  4. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  5. Molina M, et al. 2015. PLoS One. 10:127262. PubMed
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  12. Dudek M, et al. 2015. Mucosal Immunol. . PubMed
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  14. Stein K, et al. 2016. J Leukoc Biol. 99: 231 - 239. PubMed
  15. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
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  17. Saha S, et al. 2016. Nat Commun. 7:13096. PubMed
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  19. Geng S, et al. 2016. Nat Commun. 7:13436. PubMed
  20. Wang X, et al. 2016. J Immunol. 197(12):4750-4761. PubMed
  21. Zhang T, et al. 2020. JCI Insight. 5:00. PubMed
  22. Alshetaiwi H, et al. 2020. Sci Immunol. 5:00. PubMed
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  24. Runyan CE, et al. 2020. Aging Cell. 19:00. PubMed
  25. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  26. Nakayama Y, et al. 2020. Proc Natl Acad Sci U S A. 117:14365. PubMed
  27. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
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  35. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  36. Ratitong B, et al. 2021. Cell Reports. 35(7):109139. PubMed
  37. Gonçalves NP, et al. 2017. J Neuroinflammation. 14:115. PubMed
  38. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  39. Golan K et al. 2018. Cell stem cell. 23(4):572-585 . PubMed
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  41. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
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RRID
AB_1732093 (BioLegend Cat. No. 128017)
AB_1732082 (BioLegend Cat. No. 128018)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/09/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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