PE/Cyanine7 anti-mouse CD83 Antibody

Pricing & Availability
Clone
Michel-19 (See other available formats)
Regulatory Status
RUO
Other Names
HB15
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
Michael-19_PECy7_CD83_Antibody_FC_100615
Mouse CD83 transfected cells were stained with CD83 (clone Michel-19) PE/Cyanine7 (filled histogram), or rat IgG1, κ PE/Cyanine7 isotype control (open histogram).
  • Michael-19_PECy7_CD83_Antibody_FC_100615
    Mouse CD83 transfected cells were stained with CD83 (clone Michel-19) PE/Cyanine7 (filled histogram), or rat IgG1, κ PE/Cyanine7 isotype control (open histogram).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
121517 25 µg £86.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
121518 100 µg £209.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD83 is a 45 kD type I transmembrane protein. It belongs to immunoglobulin superfamily and is expressed on mature dendritic cells and activated lymphocytes. CD83 is involved in the regulation of T cell development and immune response. Soluble form CD83 has been reported to inhibit dendritic cell maturation and dendritic cell-mediated T cell proliferation. Murine CD83 ligand has been found on B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD83 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone - fixed frozen sections4.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Cramer SO, et al. 2000. Int. Immunol. 12:1347.
  2. Fujimoto Y, et al. 2002. Cell 108:755.
  3. Mott KR, et al. 2009. Virol J. 6:56. (FC) PubMed
  4. Roland Cl, et al. 2009. Mol Cancer Res. 8:1761. (IHC) PubMed
  5. Masuda Y, et al. 2010. Cancer Immunol Immunother. [Epub ahead of print] (FC) PubMed
  6. Tze LE, et al. 2011. J Exp Med. PubMed
  7. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
  2. He Q, et al. 2019. Jpn J Infect Dis. 73:111. PubMed
RRID
AB_2566123 (BioLegend Cat. No. 121517)
AB_2566124 (BioLegend Cat. No. 121518)

Antigen Details

Structure
Type I transmembrane protein, Ig superfamily, 45kD
Distribution

Mature dendritic cells, activated lymphocytes

Function
Regulate T cell development and immune response, soluble form CD83 inhibit dendritic cell maturation
Ligand/Receptor
CD83 ligand
Cell Type
Dendritic cells, Lymphocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Lechmann M, et al. 2005. Biochem. Biophys. Res. Commun. 329:132.
2. Kotxor N, et al. 2004. Immunobiology 209:129.
3. Leon F, et al. 2004. J. Immunol. 173:2995.
4. Cramer SO, et al. 2000. Int. Immunol. 12:1347.
5. Fujimoto Y, et al. 2002. Cell 108:755.

Gene ID
12522 View all products for this Gene ID
UniProt
View information about CD83 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/06/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account