PE/Cyanine7 anti-mouse CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
MIH7 (See other available formats)
Other Names
Programmed cell death ligand 1 (PD-L1), B7 homolog 1 (B7-H1), B7-H, B7H1, PDL1, PDCD1L1, PDCD1LG1
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
MIH7_PE_CYANINE7_CD274_Antibody_080519
C57BL/6 mouse splenocytes were stained with CD274 (B7-H1, PD-L1) (clone MIH7) PE/Cyanine7 (filled histogram) PE/Cyanine7, or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram).
  • MIH7_PE_CYANINE7_CD274_Antibody_080519
    C57BL/6 mouse splenocytes were stained with CD274 (B7-H1, PD-L1) (clone MIH7) PE/Cyanine7 (filled histogram) PE/Cyanine7, or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram).
Cat # Size Price Quantity Avail. Save
155405 25 µg £60
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155406 100 µg £178
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Description

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-L1 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions. The solution is free of unconjugated PE/Cyanine7 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The antibody MIH7 does not block other CD274 antibodies: clone MIH6 and 10F.9G2.

Additional reported applications (for the relevant formats) include: Blocking (Block)

Application References

(PubMed link indicates BioLegend citation)
  1. Sharma MD, et al. 2007. J Clin Invest. 117:2570-82. (Block)
  2. Sharma MD, et al. 2015. Sci Adv. 1:e1500845. (Block)
RRID
AB_2814064 (BioLegend Cat. No. 155405)
AB_2814065 (BioLegend Cat. No. 155406)

Antigen Details

Structure
Type 1 transmembrane protein, member of the B7 family, 40kD.
Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Function
CD274 is involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-CD1-independent manner. Its interaction with PD-1-CD1 inhibits T-cell proliferation and cytokine production.
Ligand/Receptor
PD-1 (CD279)
Cell Type
B cells, Dendritic cells, Endothelial cells, Monocytes, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immuno-Oncology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Dorand RD. 2016. Science. 353:399
  2. Khan AR, et al. 2015. Nat Commun. 6:5997
  3. Kiyasu J, et al. 2015. Blood. 126:2193
  4. Herold M, et al. 2015 J Immunol. 195:3584
  5. Buddhisa S, et al. 2015. J Immunol. 194:4413
Gene ID
60533 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/05/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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