PE/Cyanine7 anti-mouse CD253 (TRAIL) Antibody

Pricing & Availability
Clone
N2B2 (See other available formats)
Regulatory Status
RUO
Other Names
TNFSF10, TNF-related apoptosis inducing ligand, Apo-2 ligand, Apo-2L, CD253
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
N2B2_PECy7_CD253_Antibody_011518
Mouse TRAIL transfected L5718Y cells were stained with CD253 (clone N2B2) PE/Cyanine7 (filled histogram) or Rat IgG2a, ? PE/Cyanine7 isotype control (open histogram).
  • N2B2_PECy7_CD253_Antibody_011518
    Mouse TRAIL transfected L5718Y cells were stained with CD253 (clone N2B2) PE/Cyanine7 (filled histogram) or Rat IgG2a, ? PE/Cyanine7 isotype control (open histogram).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
109311 25 µg £82.00
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109312 100 µg £209.00
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Description

CD253 is a 40 kD TNF superfamily member known as TRAIL, Apo-2 ligand, and Apo-2L. TRAIL is expressed on a variety of cells, including IL-2 and IL-15 activated NK cells and activated T cells. However, it is undetectable on resting T and B cells. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. The N2B2 antibody has been reported to be useful for flow cytometric staining and blocking NK cell cytotoxicity in vitro.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TRAIL-transfected 2PK-3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of NK cell cytotoxicity1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109308).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1999. J. Immunol. 163:1906. (Block)
  2. Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
  3. Joshi PS, et al. 2006. J. Leukocyte Biol. 80:1345.
  4. Herold S, et al. 2008. J. Exp. Med. 205:3065. PubMed
  5. Iannello A, et al. 2009. J. Virol. 83:5999. PubMed
  6. Komatsu M, et al. 2003. Blood 101:3991. (Block)
  7. Taieb J, et al. 2006. Nature Med. 12:214. (Block)
  8. Gonzaga R, et al. 2011. J. Immunol. 187:6235. PubMed.
RRID
AB_2721675 (BioLegend Cat. No. 109311)
AB_2721676 (BioLegend Cat. No. 109312)

Antigen Details

Structure
TNF ligand superfamily member, approximately 40 kD
Distribution

Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells

Function
Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity
Ligand/Receptor
TRAIL receptor DR5
Cell Type
Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Kayagaki N, et al. 1999. J. Immunol. 163:1906.
2. Wiley SR, et al. 1995. Immunity. 3:673.
3. Wu GS, et al. 1999. Cancer Res. 59:2770.
4. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.

Gene ID
22035 View all products for this Gene ID
UniProt
View information about CD253 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01/15/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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