PE/Cyanine7 anti-mouse CD206 (MMR) Antibody

Pricing & Availability
Clone
C068C2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), MRC1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
C068C2_PE-Cy7_CD206_Antibody_FC_1_062613
Thioglycollate-elicited BALB/c mouse peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom).
  • C068C2_PE-Cy7_CD206_Antibody_FC_1_062613
    Thioglycollate-elicited BALB/c mouse peritoneal macrophages were surface stained with CD107b (Mac-3) PE, and then intracellularly stained with CD206 (clone C068C2) PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom).
  • C068C2_PE-Cy7_CD206_Antibody_FC_2_062613
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
141719 25 µg £92.00
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141720 100 µg £209.00
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Description

CD206, also known as mannose receptor (MR), is a 175 kD type I membrane protein. It is a pattern recognition receptor (PRR) belonging to the C-type lectin superfamily. MR is expressed on macrophages, dendritic cells, Langerhans cells, and hepatic or lymphatic endothelial cells. MR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine through its C-type lectin-like carbohydrate recognition domains, sulfated carbohydrate antigens through its cysteine-rich domain, and collagens through its fibronectin type II domain. MR mediates endocytosis and phagocytosis as well as activation of macrophages and antigen presentation. It plays an important role in host defense and provides a link between innate and adaptive immunity. Recently, MR on lymphatic endothelial cells was found to be involved in leukocyte trafficking and a contributor to the metastatic behavior of cancer cells. It suggests that MR may be a potential target in controlling inflammation and cancer metastasis by targeting the lymphatic vasculature.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD206 (MMR)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone C068C2 recognizes a region similar to clone MR5D3, based on the ability of the clones to block each other.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Keller J, et al. 2012. Biochem Biophys Res Commun. 417:217. PubMed
  2. Ito H, et al. 2012. J Am Soc Nephrol. 23:1797. PubMed
  3. Yang X, et al. 2015. PNAS. 112:2900. PubMed
Product Citations
  1. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  2. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  3. Ravussin A, et al. 2018. Cell Rep. 24:1085. PubMed
  4. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  5. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  6. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  7. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  8. Heinen A, et al. 2019. Mol Ther. 27:46:00. PubMed
  9. Zhang L, et al. 2019. Front Immunol. 1.381944444. PubMed
  10. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  11. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  12. Peng L, et al. 2020. Transl Lung Cancer Res. 0.892361111. PubMed
  13. Al Mamun A, et al. 2019. Neurochem Int. 127:148. PubMed
  14. Sagnella SM, et al. 2020. Cancer Cell. 37(3):354-370. PubMed
  15. Weinstock NI, et al. 2020. Neuron. 107(1):65-81. PubMed
  16. Coronel MM, et al. 2020. Sci Adv. 6:eaba5573. PubMed
  17. Scholz A, et al. 2016. EMBO Mol Med. 8: 39 - 57. PubMed
  18. Upadhya R, et al. 2016. MBio. 7: 00547-16. PubMed
  19. Brestoff JR, et al. 2021. Cell Metab. 33:270. PubMed
  20. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  21. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  22. Zheng W, et al. 2021. Wellcome Open Research. 6:52. PubMed
  23. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  24. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
  25. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
RRID
AB_2562247 (BioLegend Cat. No. 141719)
AB_2562248 (BioLegend Cat. No. 141720)

Antigen Details

Structure
Type I transmembrane protein, 175 kD, C-type lectin superfamily
Distribution

Macrophages, dendritic cells, Langerhans cells, liver endothelial cells

Function
Pathogen recognition, endocytosis and phagocytosis, antigen presentation
Ligand/Receptor
Antigen containing mannose, fucose, or an N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Langerhans cells, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
2. Apostolopoulos V, et al. 2001. Curr. Mol. Med. 1:469.
3. Burgdorf S, et al. 2006. J. Immunol. 176:6770.
4. McKenzie EJ, et al. 2007. J. Immunol. 178:4975.

Gene ID
17533 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

Why is mouse CD206 stained intracellularly and not via surface staining?

Typically, mouse CD206 surface level is relatively low under normal conditions and so intracellular staining protocol is required to get better signal.

Go To Top Version: 2    Revision Date: 04/04/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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