PE/Cyanine7 anti-mouse CD196 (CCR6) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Armenian Hamster

Immunogen

N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Product Citations

1. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
2. Boccasavia VL, et al. 2021. Cell Reports. 34(11):108861. PubMed
3. Withers D, et al. 2016. Nat Med. 22:319-23. PubMed
4. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
5. Emgård J, et al. 2018. Immunity. 143:419. PubMed
6. Marks KE, et al. 2021. Cell Reports. 35(13):109303. PubMed
7. Zanvit P, et al. 2015. Nat Commun. 6: 8424. PubMed
8. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
9. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
10. Nikolouli E, et al. 2020. Cell Mol Immunol. . PubMed
11. Sakamoto K, et al. 2022. STAR Protoc. 3:101052. PubMed

RRID

AB_1877244 (BioLegend Cat. No. 129815)
AB_2072798 (BioLegend Cat. No. 129816)

Antigen Details

Structure

G-protein linked, seven transmembrane, domain-spanning chemokine receptor; 367 amino acids long and about 75% homology with human CCR6

Distribution

Expressed on spleen B cells, immature splenic dendritic cells in Peyer's patches, and some T cell subset

Function

Important in regulating mucosal immune response

Ligand/Receptor

CCL20/MIP-3a

Cell Type

B cells, Dendritic cells, T cells

Biology Area

Immunology

Molecular Family

CD Molecules, Cytokine/Chemokine Receptors, GPCR

Antigen References

1. Rossi D, et al. 1997. J. Immunol. 158:1033.
2. Lukacs N, et al. 2001. J. Exp. Med. 194:551.
3. Cook DN, et al. 2000. Immunity 12:495

Gene ID

12458 View all products for this Gene ID

UniProt

View information about CD196 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Other Formats

Certificate of Analysis