PE/Cyanine7 anti-human CD5 Antibody

Pricing & Availability
Clone
UCHT2 (See other available formats)
Regulatory Status
RUO
Workshop
III 518
Other Names
Leu-1, Ly-1, T1, Tp67
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
UCHT2_PECy7_011909.jpg
Human peripheral blood lymphocytes were stained with CD5 (UCHT2) PE/Cyanine7 (red line) or mouse IgG1, κ PE/Cyanine7 (green line).
  • UCHT2_PECy7_011909.jpg
    Human peripheral blood lymphocytes were stained with CD5 (UCHT2) PE/Cyanine7 (red line) or mouse IgG1, κ PE/Cyanine7 (green line).
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
300621 25 tests £117
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300622 100 tests £238
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Description

CD5 is a 67 kD single chain type I glycoprotein also known as Leu-1, Ly-1 and T1. It is a member of the scavenger receptor superfamily found on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-CLL), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions upon binding to ligands such as CD72.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Capuchin Monkey, Common Marmoset, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting2 and immunohistochemical staining of acetone-fixed frozen sections2,5.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
  2. Renaudineau Y, et al. 2005. Blood 106:2781. (WB IHC)
  3. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437.
  4. Saliba AE, et al. 2010. P. Natl. Acad. Sci. USA 107:14524. PubMed
  5. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
Product Citations
  1. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  2. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  3. Jama B, Morris G 2014. J Vis Exp. 93: 52257. PubMed
  4. Bachireddy P, et al. 2020. Sci Transl Med. :12. PubMed
  5. Hu EY, et al. 2021. Blood Adv. 5:3152. PubMed
  6. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  7. Inui M, et al. 2015. Int Immunol. 27: 345 - 355. PubMed
  8. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
RRID
AB_1027696 (BioLegend Cat. No. 300621)
AB_2275812 (BioLegend Cat. No. 300622)

Antigen Details

Structure
Scavenger receptor superfamily, 67 kD
Distribution

T cells, thymocytes, B cell subset, B cell CLL, peripheral blood dendritic cells

Function
Modulates TCR, BCR signaling, thymocyte maturation, T-B cell interaction
Ligand/Receptor
CD72
Cell Type
B cells, Dendritic cells, Leukemia, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Kipps T. 1988. Adv. Immunol. 47:117.
2. Resnick D, et al. 1993. Trends Biochem. Sci. 19:5.
3. Wood GS, et al. 1992. Am. J. Pathol. 14:789.

Gene ID
921 View all products for this Gene ID
UniProt
View information about CD5 on UniProt.org
Go To Top Version: 2    Revision Date: 12/15/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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