PE/Cy7 anti-mouse CD196 (CCR6) Antibody

Pricing & Availability
Clone
29-2L17 (See other available formats)
Other Names
CD196, BN-1, CKR-L3, DRY6, KY411, STRL22
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
A_29-2L17_PECy7_111209
C57BL/6 mouse splenocytes stained with rat IgG2a PE/Cy7 isotype and CD45R/B220 (RA3-6B2) APC
  • A_29-2L17_PECy7_111209
    C57BL/6 mouse splenocytes stained with rat IgG2a PE/Cy7 isotype and CD45R/B220 (RA3-6B2) APC
  • B_29-2L17_PECy7_100609
    C57BL/6 mouse splenocytes stained with rat IgG2a PE/Cy7 isotype and CD45R/B220 (RA3-6B2) APC
See PE/Cy7 spectral data
Cat # Size Price Quantity Avail. Save
129815 25 µg £101
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129816 100 µg £218
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Description

CCR6 is a G-protein linked chemokine receptor which binds the chemokine CCL20/MIP-3a. It is expressed on B lymphocytes and certain subsets of dendritic cells and T cells. CCR6 is reported to be involved in mucosal immune response and lymphocyte migration and homeostasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
N-terminal peptide (aa. 2-38) of mouse CCR6 fused with GST
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Product Citations
  1. Zanvit P, et al. 2015. Nat Commun. 6: 8424. PubMed
  2. Withers D, et al. 2016. Nat Med. 22:319-23. PubMed
  3. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  4. Suan D, et al. 2017. Immunity. 47:1142. PubMed
Publication Library
RRID
AB_1877244 (BioLegend Cat. No. 129815)
AB_2072798 (BioLegend Cat. No. 129816)

Antigen Details

Structure
G-protein linked, seven transmembrane, domain-spanning chemokine receptor; 367 amino acids long and about 75% homology with human CCR6
Distribution

Expressed on spleen B cells, immature splenic dendritic cells in Peyer's patches, and some T cell subset

Function
Important in regulating mucosal immune response
Ligand/Receptor
CCL20/MIP-3a
Cell Type
B cells, Dendritic cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Rossi D, et al. 1997. J. Immunol. 158:1033.
2. Lukacs N, et al. 2001. J. Exp. Med. 194:551.
3. Cook DN, et al. 2000. Immunity 12:495

Gene ID
12458 View all products for this Gene ID
UniProt
View information about CD196 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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