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PE anti-Streptavidin Antibody

Pricing & Availability
Clone
3A20.2 (See other available formats)
Regulatory Status
RUO
Other Names
SAv
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
3A20.2_PE_Streptavidin_Antibody_030116
C57BL/6 thymocytes were sequentially stained with CD4 Biotin, Streptavidin purified, anti-Streptavidin (clone 3A20.2) PE antibody (filled histogram), or mouse IgG2b, κ PE isotype control (open histogram).
  • 3A20.2_PE_Streptavidin_Antibody_030116
    C57BL/6 thymocytes were sequentially stained with CD4 Biotin, Streptavidin purified, anti-Streptavidin (clone 3A20.2) PE antibody (filled histogram), or mouse IgG2b, κ PE isotype control (open histogram).
See PE spectral data
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410503 25 µg £55
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410504 100 µg £147
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Description

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including flow cytometry, molecular biology, and bionanotechnology.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Streptavidin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 3A20.2 purified antibody is useful as a third step reagent to amplify dim or low signals in many applications. This antibody does not cross-react with avidin.

Product Citations
  1. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
  2. Silvestre-Roig C, et al. 2019. Nature. 569:236. PubMed
  3. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  4. Iwabuchi R, et al. 2018. Front Immunol. 9:1042. PubMed
  5. Niitsuma S, et al. 2020. Int Immunol. 283:32. PubMed
  6. Oberhardt V, et al. 2021. Nature. 597:268. PubMed
  7. Wang Y, et al. 2022. Front Immunol. 13:847581. PubMed
  8. Shi H, et al. 2020. Immunity. 51(6):1012-1027.e7.. PubMed
  9. Wells AC et al. 2017. eLife. 6 pii: e26398. PubMed
  10. Shen B, et al. 2019. Elife. 8:e42274. PubMed
  11. Shi H et al. 2018. Immunity. 49(5):899-914 . PubMed
RRID
AB_2571914 (BioLegend Cat. No. 410503)
AB_2571915 (BioLegend Cat. No. 410504)

Antigen Details

Structure
52.8 kD tetrameric protein.
Function
Streptavidin binds to biotin with high affinity.
Ligand/Receptor
Biotin
Antigen References

1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal Methods Chem. 58:1093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.
 

Gene ID
NA
UniProt
View information about Streptavidin on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03/07/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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