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PE anti-STAT1 Phospho (Ser727) Antibody

Pricing & Availability
Clone
A15158B (See other available formats)
Regulatory Status
RUO
Other Names
Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A15158B_PE_STAT1_Antibody_1_062216
HeLa cells were stimulated with (filled histogram) or without (open histogram) nocodozole for 24 hours, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) PE.
  • A15158B_PE_STAT1_Antibody_1_062216
    HeLa cells were stimulated with (filled histogram) or without (open histogram) nocodozole for 24 hours, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) PE.
  • A15158B_PE_STAT1_Antibody_2_062216
    Human peripheral blood lymphocytes were stimulated with (filled histogram) or without (open histogram) Cell Activation Cocktail (without Brefeldin A) for 15 minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) PE.
  • A15158B_PE_STAT1_Antibody_3_062216
    NIH/3T3 cells were treated with (filled histogram) or without (open histogram) nocodozole for 24 hours, fixed with Fixation Buffer (Cat No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat No. 425401), then intracellularly stained with anti-STAT1 Phospho (Ser727) antibody (clone A15158B) PE.
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686403 25 tests £126
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686404 100 tests £288
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Description

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human STAT1 peptide phosphorylated at Ser 727.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

For staining mouse cells a higher volume than 5 µl may be required.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  2. Sanmarco LM, et al. 2021. Nature. 590:473. PubMed
  3. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  4. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
RRID
AB_2616938 (BioLegend Cat. No. 686403)
AB_2616939 (BioLegend Cat. No. 686404)

Antigen Details

Structure
750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.
Distribution

Translocates to the nucleus when phosphorylated.

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
Interaction
Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Nuclear Markers, Phospho-Proteins
Antigen References

1. Durbin JE, et al. 1996. Cell. 84:443.
2. Darnell JE Jr, et al. 1994. Science 264:1415.
3. Chen X, et al. 1998. Cell. 93:827.
4. Ramana CV, et al. 2000. Oncogene. 19:2619.

Gene ID
6772 View all products for this Gene ID
UniProt
View information about STAT1 Phospho Ser727 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06/22/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • Alexa Fluor® 488 anti-STAT1 Phospho (Ser727)

  • PE/Cyanine7 anti-STAT1 Phospho (Ser727)

  • PerCP/Cyanine5.5 anti-STAT1 Phospho (Ser727)
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