PE anti-SLP76 Phospho (Ser376) Antibody

Pricing & Availability
Clone
A18001A (See other available formats)
Other Names
LCP2, Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A18001A_PE_SLP76_Antibody_1_071219.png
Human peripheral blood lymphocytes were treated with (right) or without (left) Hydrogen Peroxide for 5 minutes, fixed with Fixation Buffer (Cat No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat No. 425401), then surfaced stained with CD3 APC and intracellularly stained with anti-SL76 Phospho (Ser376) (clone A18001A) PE.
  • A18001A_PE_SLP76_Antibody_1_071219.png
    Human peripheral blood lymphocytes were treated with (right) or without (left) Hydrogen Peroxide for 5 minutes, fixed with Fixation Buffer (Cat No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat No. 425401), then surfaced stained with CD3 APC and intracellularly stained with anti-SL76 Phospho (Ser376) (clone A18001A) PE.
  • A18001A_PE_SLP76_Antibody_2_071219.png
    Human peripheral blood lymphocytes were treated with (filled histogram) or without (open histogram) cross-linked anti-human CD3 (Cat No. 300438) plus anti-human CD28 (Cat No. 302914) (10 µg/ml each, 2 min), fixed with Fixation Buffer (Cat No. 420801), permeabilized with Intracellular Permeabilization Wash Buffer (Cat No. 421002), then surface stained with CD3 and CD20 and intraceullarly stained with anti-SLP76 Phospho (Ser376) (clone A18001A) PE. Cells were gated on CD3+CD20-.
  • A18001A_PE_SLP76_Antibody_3_071219.png
    Human peripheral blood lymphocytes were treated with (filled histogram) or without (open histogram) cross-linked anti-human CD3 (Cat No. 300438) plus anti-human CD28 (Cat No. 302914) (10 µg/ml each, 2 min), fixed with Fixation Buffer (Cat No. 420801), permeabilized with Intracellular Permeabilization Wash Buffer (Cat No. 421002), then surface stained with CD3 and CD20 and intraceullarly stained with anti-SLP76 Phospho (Ser376) (clone A18001A) PE (Figures D and E). Cells were gated on CD3-CD20+.
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Cat # Size Price Quantity Avail. Save
667103 25 tests £87
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667104 100 tests £215
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Description

SLP76 is a cytosolic adapter protein that functions as a substrate in the T cell receptor (TCR) activation pathway. Upon TCR engagement, SLP76 is phosphorylated on multiple residues, resulting in the protein being tranlocated to the plasma membrane and facilitating the assembly of a multi-subunit complex comprised of Vav1, Nck, ITK, and PLCγ1. SLP76 also plays an essential role in normal T cell development.

SLP76 also represents a negative regulatory node in the TCR activation pathway. Following TCR engagement, SLP76 is phosphorylated at Ser376 by the hematopoietic progenitor kinase (HPK1). This modification promotes an interaction between SLP76 and the scaffolding protein 14-3-3ε, resulting in dissociation of the TCR signaling complex and a diminution of TCR signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic human SLP76 peptide containing phosphorylated Ser376
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone was tested for ICC on PFA-fixed and methanol permeabilized Jurkat cells treated with or without H2O2 and produced nuclear staining that was not responsive to the treatment. We therefore do not recommend this clone for ICC.

cross-reactivity to Mouse confirmed based on Western blot testing.

Antigen Details

Structure
SLP76 is a 533 amino acid protein with a predicted molecular weight of ~60 kDa.
Distribution

Hematopoietic cells/cytosol

Function
Adapter protein/TCR Signaling
Interaction
ITK, LAT, GRB2, PLCγ1, NCK, VAV, ITK, LCK, 14-3-3ε
Cell Type
B cells, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References
  1. Sauer K, et al. 2001. J Biol Chem. 276:45207.
  2. Koretzky GA, et al.. 2006. Nat Rev Immunol. 6:67.
  3. Di Bartolo V, et al. 2007. J Exp Med. 204:681.
  4. Lasserre, et al. 2011. J Cell Biol. 195:839.

 

Gene ID
3937 View all products for this Gene ID
UniProt
View information about SLP76 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/12/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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