PE anti-rat CD45RA Antibody

Pricing & Availability
OX-33 (See other available formats)
Regulatory Status
Other Names
Leukocyte common antigen, high molecular weight form
Mouse IgG1, κ
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Product Citations
LOU rat splenocytes stained with OX-33 PE
  • OX-33_PE_021308
    LOU rat splenocytes stained with OX-33 PE
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202307 50 µg £97
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CD45 is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets. CD45RA is one of the CD45 isoforms with a molecular weight of 200-220 kD. It is expressed almost exclusively on B cells. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins, including galectin-1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Leukocyte common antigen purified from rat splenocytes.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1.

Application References

(PubMed link indicates BioLegend citation)
  1. Smith T, et al. 2000. Nature Med. 6:62. (IHC)
Product Citations
  1. Kumar P et al. 2015. Journal of neuroinflammation. 12:22 . PubMed
  2. Winkler A, et al. 2021. J Clin Invest. 131:. PubMed
  3. Chen C, et al. 2018. J Neuroinflammation. 15:167. PubMed
  4. Wang Y, et al. 2012. PLoS One. 7:e43825. PubMed
  5. Krashia P et al. 2019. Nat Commun. 10(1):3945 . PubMed
  6. Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed
AB_314010 (BioLegend Cat. No. 202307)

Antigen Details

Protein tyrosine phosphatase, type I transmembrane protein, hign molecular weight isoform approximately 200-220 kD

Primarily on B lymphocytes

B cell receptor signal transduction
Galectin-1, CD2, CD3, CD4
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Sunderland CA, et al. 1979. Eur. J. Immunol. 9:155.
2. Woolett GR, et al. 1985. Eur. J. Immunol. 15:168.

Gene ID
24699 View all products for this Gene ID
View information about CD45RA on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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