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PE anti-mouse TCR Vδ6.3 Antibody

Pricing & Availability
Clone
C504.17C (See other available formats)
Regulatory Status
RUO
Other Names
TCRd, TCR-delta
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
C50417C_PE_TCRVS63_Antibody_022818
C57BL/6 splenocytes were stained with APC anti-mouse TCR γ/δ (clone GL3); these cells were then stained with PE Anti-Mouse TCR vδ6.3 (clone C504.17C; left) or PE Rat IgG2b, κ Isotype Ctrl (right) followed by FITC anti-mouse CD3ε. Data shown were gated on the CD3ε positive population.
  • C50417C_PE_TCRVS63_Antibody_022818
    C57BL/6 splenocytes were stained with APC anti-mouse TCR γ/δ (clone GL3); these cells were then stained with PE Anti-Mouse TCR vδ6.3 (clone C504.17C; left) or PE Rat IgG2b, κ Isotype Ctrl (right) followed by FITC anti-mouse CD3ε. Data shown were gated on the CD3ε positive population.
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154803 25 µg £89
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154804 100 µg £184
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Description

Lymphocytes expressing γ/δ antigen receptors constitute a minor population in lymphoid tissue, but a major population within epidermal, gastrointestinal, and vaginouterine epithelia. γ/δ T cells are involved in immunological surveillance and regulation in these tissues. The C504.17C antibody reacts with Vδ6.3 T-cell receptor (TCR) bearing T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TCR γδ positive T Cell Hybridoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

If clone GL3 is included in multi-color analysis, it is recommended that the cells should be stained with clone GL3 first prior to be stained with clone C504.17C.

Application References

(PubMed link indicates BioLegend citation)
  1. Roark CE, et al. 1993. J Immunol. 150:4867.
RRID
AB_2728218 (BioLegend Cat. No. 154803)
AB_2728219 (BioLegend Cat. No. 154804)

Antigen Details

Structure
Form heterodimer with TCR gamma chain, associate with CD3 complex.
Distribution

Expressed on Vδ6.3 T-cell receptor (TCR) bearing γ/δ T cells

Function
Involved in immunological surveillance and regulation.
Ligand/Receptor
Antigens presented on MHC or MHC-like molecules
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References
  1. Raulet DH. 1989. Annu Rev Immunol. 7:175.
  2. Allison JP and Havran WL. 1991. Annu Rev Immunol. 9:679.
Gene ID
110066 View all products for this Gene ID
UniProt
View information about TCR Vdelta6.3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02/28/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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