PE anti-mouse Podoplanin Antibody

Pricing & Availability
Clone
8.1.1 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, gp38, stromal cell marker
Isotype
Syrian Hamster IgG
Ave. Rating
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Product Citations
publications
8_1_1_PE_081908.jpg
TE-71, mouse thymic epithelial stromal cell line, stained with 8.1.1 PE
  • 8_1_1_PE_081908.jpg
    TE-71, mouse thymic epithelial stromal cell line, stained with 8.1.1 PE
Compare all formats See PE spectral data
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127407 50 µg £83
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127408 200 µg £253
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Description

The mucin-type glycoprotein podoplanin is thought to be involved in the development of the lymphatic vascular system. Podoplanin is named after its expression in the kidney glomerular epithelial cells (podocytes). It has a potential role in tumor progression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry6, and spatial biology (IBEX)8,9.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
  2. Farr AG, et al. 1992. J. Exp. Med. 176:1477.
  3. Bekiaris V, et al. 2008. J. Immunol. 180:6768.
  4. Algars A, et al. 2011. Blood 117:4387. PubMed
  5. Reis VO, et al. 2012. Immunobiology. 217:831. PubMed
  6. Kaji C, et al. 2012. Acta. Histochem. Cytochem. 45:227. (IHC)
  7. Kretschmer S, et al. 2013. PLoS One. 8:e52201. PubMed.
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  2. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  3. Onder L et al. 2017. Immunity. 47(1):80-92 . PubMed
  4. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  5. Bresson L, et al. 2018. Development. 145. PubMed
  6. Guérin MV, et al. 2019. Nat Commun. 10:4131. PubMed
  7. Karpus ON, et al. 2019. Cell Rep. 26:3698. PubMed
  8. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  9. Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
  10. Peranzoni E, et al. 2018. Proc Natl Acad Sci U S A. 115:E4041. PubMed
  11. Kunimura K, et al. 2020. Cell Reports. 29(9):2823-2834.e7.. PubMed
  12. Novkovic M, et al. 2020. Cell Reports. 30(3):893-904.e6.. PubMed
  13. Hara T, et al. 2012. J Immunol. 189:1577. PubMed
  14. Mkrtichyan M, et al. 2012. J Immunol. 189:2450. PubMed
  15. Zinselmeyer B, et al. 2013. J Exp Med. 210:757. PubMed
  16. Othy S, et al. 2013. J Immunol. 190:4535. PubMed
  17. Ito K, et al. 2014. Proc Natl Acad Sci U S A. 111:3080. PubMed
  18. Sullivan B, et al. 2015. PLoS Pathog. 11:1004588. PubMed
  19. Sun Q, et al. 2015. Bone Res. 3: 15026. PubMed
  20. Mishra A, et al. 2016. J Immunol. 197: 2828 - 2837. PubMed
  21. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  22. Francipane MG, et al. 2020. Am J Pathol. 190:252. PubMed
  23. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  24. Baasch S, et al. 2021. Cell. . PubMed
RRID
AB_2161929 (BioLegend Cat. No. 127407)
AB_2161928 (BioLegend Cat. No. 127408)

Antigen Details

Structure
43 Kd glycosylated type-1 transmembrane protein. Mucin-type protein.
Distribution

Expressed on epithelial and mesothelial cells.

Cell Type
Epithelial cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Farr A, et al. 1992. J. Histochem. Cytochem. 40:651.
2. Schacht V, et al. 2005. Am. J. Pathol. 166:913.

Gene ID
14726 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/03/2017

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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