PE anti-mouse CXCL9 (MIG) Antibody

Pricing & Availability
Clone
MIG-2F5.5 (See other available formats)
Regulatory Status
RUO
Other Names
MIG-1, MIG
Isotype
Armenian Hamster IgG, κ
Ave. Rating
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Product Citations
publications
MIG-2F5_PE_012110
IFN-g-primed (2 hour) and LPS-stimulated (overnight) Balb/c peritoneal macrophages intracellularly stained with MIG-2F5 PE
  • MIG-2F5_PE_012110
    IFN-g-primed (2 hour) and LPS-stimulated (overnight) Balb/c peritoneal macrophages intracellularly stained with MIG-2F5 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
515603 25 µg £120.00
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515604 100 µg £239.00
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Description

MIG, also known as mig-1, CXCL9, is a member of the alpha subfamily of inflammatory chemokine. It is inducible in macrophages, hepatocytes, and endothelial cells by IFN-γ, but not by TNF-α or bacterial lipopolysacchrides (LPS). Mig functions as a chemotactic factor for resting memory and activated T cells, both CD4+ and CD8+, and natural killer cells. Furthermore, it was reported that Mig induced both calcium signals and chemotaxis in activated B cells and that B cell activation induced expression of mouse CXCR3. MIG and CXCR3 may be important not only to recruit T cells to peripheral inflammatory sites, but also in some cases to maximize interactions among activated T cells, B cells, and dendritic cells within lymphoid organs to provide optimal humoral responses to pathogens.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  2. Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
  3. Asai A, et al. 2010. Infect Immun. 78:4311. PubMed
  4. Johnson S, et al. 2015. J Virol. 89: 7494-7505. PubMed
  5. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  6. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
RRID
AB_2245490 (BioLegend Cat. No. 515603)
AB_2245489 (BioLegend Cat. No. 515604)

Antigen Details

Structure
A member of the alpha subfamily of inflammatory chemokine.
Distribution
Inducible in macrophages, hepatocytes, and endothelial cells by IFN-γ.
Function
Recruit T cells to peripheral inflammatory sites, maximize interactions among activated T cells, B cells, and dendritic cells within lymphoid organs.
Ligand/Receptor
CXCR3
Cell Type
Macrophages
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Thapa M et al. 2008. J. Immunol. 180(2):1098
2. Whiting D et al. 2004. J. Immunol. 172 (12):7417
3. Helbig KJ. et al. 2009. J Virol. 83(2):836

Gene ID
4283 View all products for this Gene ID
UniProt
View information about CXCL9 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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