PE anti-mouse CD45RB Antibody

Pricing & Availability
Clone
MB4B4 (See other available formats)
Regulatory Status
RUO
Other Names
CD45RB
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
MB4B4_APE_CD45RB_Antibody_031116
C57BL/6 mouse splenocytes were stained with CD45RB (clone MB4B4) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • MB4B4_APE_CD45RB_Antibody_031116
    C57BL/6 mouse splenocytes were stained with CD45RB (clone MB4B4) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Avail. Save
151603 50 µg £41.00
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151604 200 µg £123.00
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Description

CD45RB is a 220 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes/macrophages, granulocytes, and dendritic cells. CD45RB plays a critical role in TCR and BCR signaling. As T cells become activated and progress from naïve to memory cells, CD45RB expression is downregulated. Additionally, functionally distinct CD4+ T cell subsets, which secrete differing cytokine profiles, can be separated by CD45RB intensity. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4 and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
anti-Ig activated murine B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Birkeland ML, et al. 1988. J. Mol. Cell. Immunol. 4:71.
  2. Birkeland ML, et al. 1989. Proc. Natl. Acad. Sci. USA 86:6734.
  3. Lazarovits AI, et al. 1999. Kidney Int. 55:1303.
RRID
AB_2572033 (BioLegend Cat. No. 151603)
AB_2572034 (BioLegend Cat. No. 151604)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane, 220 kD (exon 5 splicing of CD45 gene).
Distribution

Thymocytes, T cell subset, B cells, monocytes/macrophages, granulocytes, and dendritic cells.

Function
Enhances TCR and BCR signaling.
Ligand/Receptor
Galectin-1, CD2, CD3, and CD4.
Cell Type
Thymocytes, T cells, B cells, Monocytes, Macrophages, Dendritic cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Lee KM, et al. 2014. Cell Transplant. 23:51.
2. Kamanaka M, et al. 2011. J. Exp. Med. 208:1027.
3. Gagliani N, et al. 2011. PLoS One 6(12):e28434.
4. Ten Hove T, et al. 2004. J. Leuko. Biol. 75:1010.
5. Ogilvy S, et al. 2003. J. Immunol. 171:1792.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45RB on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 03/11/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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