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PE anti-mouse CD24 Antibody

Pricing & Availability
Clone
30-F1 (See other available formats)
Regulatory Status
RUO
Other Names
Heat Stable Antigen (HSA), HsAg, Ly-52, Nectadrin
Isotype
Rat IgG2c, κ
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Product Citations
publications
30-F1_PE_052710
C57BL/6 mouse splenocytes stained with 30-F1 PE
  • 30-F1_PE_052710
    C57BL/6 mouse splenocytes stained with 30-F1 PE
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138503 50 µg £59
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138504 200 µg £167
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Description

CD24 is a 35-45 kD protein, also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells, hematopoietic stem cells, and neurons. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus/spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (FC)
Product Citations
  1. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  2. Bommireddy R, et al. 2020. Vaccines (Basel). 8:. PubMed
  3. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  4. Cong M, et al. 2021. Methods Mol Biol. 2388:59. PubMed
  5. Upadhya R, et al. 2016. MBio. 7: 00547-16. PubMed
  6. Bradley T et al. 2018. Cell reports. 25(1):107-117 . PubMed
  7. Kishi Y, et al. 2021. STAR Protocols. 2(2):100540. PubMed
  8. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
RRID
AB_10576359 (BioLegend Cat. No. 138503)
AB_10578416 (BioLegend Cat. No. 138504)

Antigen Details

Structure
GPI-linked sialoglycoprotein, 35-45 kD.
Distribution

Lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells, and neurons

Function
Regulate B and T cell proliferation and differentiation.
Ligand/Receptor
P-selectin, CD24
Cell Type
B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Lymphocytes, Monocytes, Neurons, Thymocytes
Biology Area
Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Li YS, et al. 1993. J. Exp. Med. 178:951.
2. Aigner S, et al. 1995. Int. Immunol. 7:1557.
3. Hunte BE, et al. 1998. Eur. J. Immunol.. 28:3850.
4. Auerbach R, et al. 1996. Stem Cells 14:269.
5. Allman DM, et al. 1993. J. Immunol.. 151:4431.

Gene ID
12484 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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