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PE anti-mouse CD200R3 Antibody

Pricing & Availability
Clone
Ba13 (See other available formats)
Regulatory Status
RUO
Other Names
CD200 R3, CD200RLb, OX-2 Receptor 3, CD200 receptor 3
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
Ba13_PE_1_102711
C57BL/6 peripheral blood leukocytes were stained with FcεRIα APC and CD200R3 (clone Ba13) PE (top) or rat IgG2a PE isotype control (bottom). Data shown were gated on lymphocyte population.
  • Ba13_PE_1_102711
    C57BL/6 peripheral blood leukocytes were stained with FcεRIα APC and CD200R3 (clone Ba13) PE (top) or rat IgG2a PE isotype control (bottom). Data shown were gated on lymphocyte population.
  • Ba13_PE_2_102711
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142205 25 µg £92
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142206 100 µg £238
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Description

CD200R3, also known as CD200RLb and OX-2 Receptor 3, is a disulfide-linked dimeric CD200R-like receptor which belongs to immunoglobulin superfamily.  Its positively charged amino acid lysine associates with ITAM- or YxxM motif-bearing adaptor molecules such as DAP12, DAP10, FcRγ, and CD3ζ. CD200R3 functions as an activating receptor to regulate IgE independent immune response.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse primary basophils
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Ba13 recognizes circulating and bone marrow basophils; it also recognizes a subset of mast cells in the peritoneal cavity and skin. Additional reported applications (for the relevant formats) include: stimulation of bone marrow derived basophils to produce IL-4.

Product Citations
  1. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  2. Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed
  3. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  4. de Reuver R, et al. 2021. Cell Reports. 36(6):109500. PubMed
  5. Zhang X, et al. 2020. Arterioscler Thromb Vasc Biol. 40:597. PubMed
  6. Dietschmann A, et al. 2020. Eur J Immunol. 50:1044. PubMed
  7. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
RRID
AB_10915271 (BioLegend Cat. No. 142205)
AB_10916520 (BioLegend Cat. No. 142206)

Antigen Details

Structure
Immunoglobulin superfamily; disulfide-linked dimer with positively charge amino acids in transmembrane; molecular weight is about ~38 kD or ~74 kD (reduced or non-reduced conditions, respectively)
Distribution

Basophils in peripheral blood, bone marrow, spleen; mast cells in peritoneal cavity and skin

Function
Transduce stimulatory signals
Interaction
DAP12, DAP10, FcRγ, or CD3ζ
Cell Type
Basophils, Mast cells
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Voehringer D, et al. 2004. J. Biol. Chem. 52:54117.
2. Kojima T, et al. 2007. J. Immunol. 179:7093.
3. Sato K, et al. 2009. Blood 113:4780.

Gene ID
74603 View all products for this Gene ID
UniProt
View information about CD200R3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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