- 5B11 (See other available formats)
- Regulatory Status
- Other Names
- IL-3 Receptor α chain, IL-3Rα
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD123 is a 70 kD α chain subunit of the IL-3 receptor (IL-3R α). It is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL-3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either the common β chain (CDw131) or the IL-3R β chain (AIC2A) to form high-affinity heterodimeric IL-3 receptors. CDw131 can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors, while the IL-3 R β subunit is specific for IL-3 but binds with low affinity. IL-3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. The 5B11 antibody does not block binding of IL-3 to the high affinity IL-3 receptor.Product Details
- Antibody Type
- Host Species
- CTLL cells transfected with the mouse IL-3Rα chain
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106004) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining.
(PubMed link indicates BioLegend citation)
- Ichihara, et al. 1995. EMBO J. 14:939. (FC)
- Mueller DL, et al. 1994. J. Immunol. 153:3014. (FC)
- Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC)
- Product Citations
AB_2124403 (BioLegend Cat. No. 106005)
- Ig superfamily, associates with CDw131 (common β chain) or IL-3 receptor β chain, 70 kD
Hematopoietic progenitors, basophils, mast cells, megakaryocytes
- Proliferation, differentiation of hematopoietic cells
- Cell Type
- Basophils, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Megakaryocytes
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Miyajima A, et al. 1993. Blood 82:1960.
3. Itoh N, et al. 1990. Science 247:324.
- Gene ID
- 16188 View all products for this Gene ID
- View information about CD123 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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