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PE anti-mouse CD120a (TNF R Type I/p55) Antibody

Pricing & Availability
Clone
55R-286 (See other available formats)
Regulatory Status
RUO
Other Names
TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A
Isotype
Armenian Hamster IgG
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Product Citations
publications
55R-286
NIH/3T3 cells stained with 55R-286 PE
  • 55R-286
    NIH/3T3 cells stained with 55R-286 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
113003 50 µg £84
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113004 200 µg £242
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Description

CD120a is a 55 kD Type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type I (TNFRI) or p55. It is expressed on a variety of cells at low levels. Resting T cells and erythrocytes express very little to no CD120a. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRADD and RIP, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli-expressed extracellular domain of the mouse TNFRI protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Flow Cytometry3,4: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113003/113004)or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).
ELISA Capture2: The 55R-286 antibody is useful as a capture antibody when paired with the biotinylated 55R-170 antibody (Cat. No. 112904) as the detecting antibody.
Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Pinckard, J.K., et al. 1997. J. Biol Chem. 272:10784. (IP)
  2. Sheehan, K.C., et al. 1995. J. Exp. Med. 181:607. (ELISA Capture)
  3. Schlaepfer, D.D., et al. 2007. J. Biol. Chem. 282:17450. (FC)
  4. Bell, J.H., et al. 2007. J. Leukocyte Biol. 82:173. (FC)
  5. Kuroishi, T., et al. 2008. J. Leukocyte Biol. 83:912.
  6. Wang K, et al. 2012. PLoS One. 7:e52924. PubMed
  7. Pereira IR, et al. 2015. PLoS Negl Trop Dis. 9:3659. PubMed
Product Citations
  1. Pereira I, et al. 2015. PLoS Negl Trop Dis. 9:3659. PubMed
  2. Vilar-Pereira G, et al. 2016. Antimicrob Agents Chemother. 60: 4297 - 4309. PubMed
  3. Pobezinskaya EL et al. 2019. Front Immunol. 1.079861111 . PubMed
  4. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  5. Li H, et al. 2018. Nat Commun. 9:2830. PubMed
  6. Habtetsion T et al. 2018. Cell metabolism. 28(2):228-242 . PubMed
  7. Wang K, et al. 2012. PLoS One. 7:e52924. PubMed
  8. Puimège L, et al. 2015. EMBO Mol Med. 7: 1004-1017. PubMed
  9. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  10. Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed
RRID
AB_313532 (BioLegend Cat. No. 113003)
AB_313533 (BioLegend Cat. No. 113004)

Antigen Details

Structure
TNFR superfamily, 55 kD
Distribution

Variety of cell types at low levels

Function
Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor
TNF-α, LT-α (TNF-β)
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Aggarwal, B. B., et al. 1985. Nature 318 665.
2. Chan, F. K.M., et al. 2000. Science 288:2351.
3. Loetscher, H., et al. 1990. Cell 61:351.
4. Rothe, J., et al. 1993. Nature 364:798.

Gene ID
21937 View all products for this Gene ID
UniProt
View information about CD120a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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