PE anti-IκB-α Antibody

Pricing & Availability
Clone
3D6C02 (See other available formats)
Other Names
Nuclear factor of kappa light chain enhancer in B cells-inhibitor alpha, IKBA
Isotype
Mouse IgG2b, κ
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Product Citations
publications
3D6C02_PE_IkBa_Antibody_122718
Human peripheral blood mononuclear cells were treated with (filled histogram) and without (dashed histogram) Cell Activation Cocktail for 15 minutes, fixed with Fixation Buffer, permeabilized with cold True-Phos™ Perm Buffer, then intracellulary stained with anti-IκB-α (clone 3D6C02) PE and anti-CD3 (UCHT1) APC. Histograms were gated off of CD3+ lymphocytes.
  • 3D6C02_PE_IkBa_Antibody_122718
    Human peripheral blood mononuclear cells were treated with (filled histogram) and without (dashed histogram) Cell Activation Cocktail for 15 minutes, fixed with Fixation Buffer, permeabilized with cold True-Phos™ Perm Buffer, then intracellulary stained with anti-IκB-α (clone 3D6C02) PE and anti-CD3 (UCHT1) APC. Histograms were gated off of CD3+ lymphocytes.
  • 3D6C02_PE_IkBa_Antibody_2_122718
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages were treated with (filled histogram) and without (dahed histogram) 100 ng/mL LPS for 15 minutes, fixed with Fixation Buffer, permeabilized with cold True-Phos™ Perm Buffer, then intracellulary stained with anti-IκB-α (clone 3D6C02) PE.
See PE spectral data
Cat # Size Price Quantity Avail. Save
662411 25 tests £91
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662412 100 tests £205
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Description

IκB-α is an inhibitory protein that binds to the NF-κB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IκB proteins are phosphorylated and degraded via the ubiquitin pathway allowing for nuclear translocation of NF-κB. The NF-κB complex can then bind DNA to activate gene expression of multiple proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Hamster
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human IκB-α recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cyanine5.5 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.0075 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This antibody weakly reacts with mouse and rat. Based on in-house testing, clone 3D6C02 does not block clone 25/IkBa/MAD-3, and only partially blocks L35A5 also raised against IκB-α.

RRID
AB_2783365 (BioLegend Cat. No. 662411)
AB_2783366 (BioLegend Cat. No. 662412)

Antigen Details

Structure
Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
Distribution

Cytoplasm

Function
Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
Interaction
NFkB1, SUMO4, p53, ribosomal S6 kinase 1
Antigen References

1. Baeuerle PA. 1998. Cell 95:729.
2. Hoffmann A, et al. 2002. Science 298:1241.
3. Ito CY, et al. 1995. Genomics 29:490.

Gene ID
4792 View all products for this Gene ID
UniProt
View information about IkappaB-alpha on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12/27/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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