PE anti-human Ki-67 Antibody

Pricing & Availability
Clone
Ki-67 (See other available formats)
Regulatory Status
RUO
Other Names
Antigen Ki-67
Isotype
Mouse IgG1, κ
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Product Citations
publications
Ki-67_PE_1_100410
Human T leukemia cell line, Jurkat, fixed and permeabilized with 70% ethanol, then intracellularly stained with Ki-67 PE and counterstained with 7-AAD (Cat No. 420404) for DNA staining.
  • Ki-67_PE_1_100410
    Human T leukemia cell line, Jurkat, fixed and permeabilized with 70% ethanol, then intracellularly stained with Ki-67 PE and counterstained with 7-AAD (Cat No. 420404) for DNA staining.
  • Ki-67_PE_3_100410
    Resting (dashed line) or PHA-activated human peripheral blood lymphocytes (day-3, solid line) fixed and permeabilized with 70% ethanol, then intracellularly stained with Ki-67 PE.
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350503 25 tests £79.00
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350504 100 tests £195.00
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Description

Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD. Both isoforms contain one forkhead-associated domain and 16 concatenated "Ki-67 repeats," each containing the epitope recognized by the mAb Ki-67. The antigen Ki-67 interacts with Hklp2, hNIFK, and chromobox protein homolog 1, 3, and 5. Ki-67 is required for cell proliferation and its expression is restricted to the phases G1, S, G2, and M of the cell cycle. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. Ki-67 has also been used to study myocyte proliferation after myocardial infarction as well as lymphocyte proliferation during infection, and has been used in neurons of patients with different neuropathologies.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Bovine
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Nuclei of the Hodgkin lymphoma cell line L428
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting3, and immunofluorescence microscopy4.

Ki-67 Staining Protocol:

1. Prepare 70% ethanol and chill at -20°C.
2. Prepare target cells of interest and wash 2X with PBS by centrifuge at 350xg for 5 minutes.
3. Discard supernatant and loosen the cell pellet by vortexing.
4. Add 3 ml cold 70% ethanol drop by drop to the cell pellet while vortexing.
5. Continue vortexing for 30 seconds and then incubate at -20°C for 1 hour.
6. Wash 3X with BioLegend Cell Staining Buffer and then resuspend the cells at the concentration of 0.5-10 x 106/ml.
7. Mix 100 µl cell suspension with proper fluorochrome-conjugated Ki-67 antibody and incubate at room temperature in the dark for 30 minutes.
8. Wash 2X with BioLegend Cell Staining Buffer and then resuspend in 0.5 ml cell staining buffer for flow cytometric analysis.

Application References

(PubMed link indicates BioLegend citation)
  1. Gerdes J, et al. 1983. Int. J. Cancer 31:13. (IHC)
  2. Gerdes J, et al. 1984. J. Immunol. 133:1710. (ICFC)
  3. Schluter C, et al. 1993 J. Cell Biol. 123:513. (IHC, WB)
  4. Bading H, et al. 1989 Exp. Cell. Res. 185:50. (IF)
  5. Guha P, et al. 2013. PNAS. 110:5052. PubMed
Product Citations
  1. Day C, et al. 2017. J Immunol. 10.4049/jimmunol.1700558. PubMed
  2. Gao Y, et al. 2018. Oncol Lett. 15:4781. PubMed
  3. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  4. Fujii S, et al. 2019. Stem Cells Dev. 28:28. PubMed
  5. Makabe K, et al. 2019. Invest Ophthalmol Vis Sci. 60:1298. PubMed
  6. Trotta E, et al. 2018. Nat Med. 24:1005. PubMed
  7. Taylor HE, et al. 2018. AIDS. 32:2847. PubMed
  8. Wangler S, et al. 2019. Spine (Phila Pa 1976). 44:1613. PubMed
  9. Hübner M, et al. 2020. Neurooncol Adv. 2:vdaa047. PubMed
  10. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  11. Perreau M, et al. 2012. J Exp Med. 210:143. PubMed
  12. Muellenbeck M, et al. 2013. J Exp Med. 210:389. PubMed
  13. Pembroke T, et al. 2014. Gut. 63:515. PubMed
  14. Liu B, et al. 2015. J Virol. 89: 11834 - 11844. PubMed
  15. Jung M, et al. 2016. Sci Signal. 9: ra64. PubMed
  16. Sugita S, et al. 2016. Stem Cell Reports. 7:619-634. PubMed
  17. Wei C, et al. 2016. Cell Death Dis. 7:e2489. PubMed
  18. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  19. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  20. Seo IH, et al. 2021. Cell Reports. 36(4):109438. PubMed
  21. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  22. Su Y, et al. 2020. Cell. 1479:183. PubMed
  23. Li M, et al. 2020. Virol Sin. 35:588. PubMed
RRID
AB_10660818 (BioLegend Cat. No. 350503)
AB_10660752 (BioLegend Cat. No. 350504)

Antigen Details

Structure
Two isoforms with molecular weights of 395 and 345 kD, one forkhead-associated domain, 16 concatenated Ki-67 repeats, located in nucleus
Distribution
Expressed in the phases G1, S, G2, and M of the cell cycle
Function
Required for cell proliferation
Interaction
Chromobox protein homolog 1, 3 and 5, Hklp2, and hNIFK
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Molecular Family
Nuclear Markers
Antigen References

1. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
2. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
3. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
4. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
5. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.

Gene ID
4288 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 07/16/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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