PE anti-human IRF3 Antibody

Pricing & Availability
Clone
AR1 (See other available formats)
Other Names
IRF3, IRF-3, interferon regulatory factor 3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_AR1_PE_IRF3_Antibody_dotplot_011419.png
Human peripheral blood lymphocytes were fixed with Fixation Buffer (Cat. No. 420801) and Permeablized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with anti-human CD3 (clone UCHT1) Pacific Blue™ and anti-human IRF3 (clone AR1) PE (bottom left) or Mouse IgG1, κ PE isotype control (bottom right).
  • 1_AR1_PE_IRF3_Antibody_dotplot_011419.png
    Human peripheral blood lymphocytes were fixed with Fixation Buffer (Cat. No. 420801) and Permeablized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with anti-human CD3 (clone UCHT1) Pacific Blue™ and anti-human IRF3 (clone AR1) PE (bottom left) or Mouse IgG1, κ PE isotype control (bottom right).
  • 2_AR1_PE_IRF3_Antibody_histogram_011419.png
    REH (low IRF3 expression) (top left) and PC-3 (high IRF3 expression) (top right) cell lines were fixed with Fixation Buffer (Cat. No. 420801) and permeabilized with Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with anti-human IRF3 (clone AR1) PE (filled histogram) or Mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Avail. Save
395903 25 tests £91
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395904 100 tests £218
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Description

IRF-3 is a roughly 50 kD member of the interferon regulatory transcription factor (IRF) family. Along with other members of the IRF family IRF-3 plays a key role in the innate immune response to viral infection. It is located ubiquitously in an inactivated form in the cytoplasm of many immune cells. When phosphorylated it forms a complex with CREBBP to upregulate many immune responses integral to innate immune function.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IRF-3 protein (entire protein)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Rustagi A, et al. 2012. Methods. 59(2):225-32

Antigen Details

Structure
Human IRF-3 has 427 amino acids with predicted Mw 47.2 kD and observed Mw 52 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9 kD and observed Mw 45 kD.
Distribution

Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.

Function
CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
Cell Type
Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Pattwell SS, et al. 2017. Trends Mol Med. (9):773-776
  2. Zhang ZX, et al. 2017. Leuk Lymphoma. 58(3): 646-654
  3. Zhao B, et al. 2016. Proc Natl Acad Sci USA. 113(24)E3403-12
Gene ID
3661 View all products for this Gene ID
UniProt
View information about IRF3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 01/07/2019

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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