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PE anti-human IFN-γ R β chain Antibody

Pricing & Availability
Clone
2HUB-159 (See other available formats)
Regulatory Status
RUO
Workshop
VI C-111
Other Names
IFN-γRβ, IFNγAF-1
Isotype
Hamster IgG
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Product Citations
publications
2HUB-159_PE_072909.jpg
Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE
  • 2HUB-159_PE_072909.jpg
    Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE
See PE spectral data
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308504 100 tests £208
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Description

IFN-γ R β chain is a 65 kD type I transmembrane protein, also known as accessory factor (AF-1) or IFN-γRII. IFN-γ receptor is composed of an IFN-γ R α chain (CDw119) and a β chain. They are members of the class II cytokine receptor family. The α chain is required for ligand binding and ligand trafficking to the cells and also plays some role in signal transduction. The β chain is critical for delivering IFN-γ mediated pleiotropic signals. IFN-γ R β chain is broadly expressed on a variety of cells at low levels and upregulated on some activated B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Hamster
Immunogen
soluable extracellular domains of the human IFN-γ R β chain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting2,3 and immunoprecipitation2,3. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). TThe LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308506).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (IP WB)
  3. Rosenzweig SD, et al. 2004. J. Immunol. 173:4000. (IP WB)
  4. Joseph TD, et al. 2001. J. Biol. Chem. 276:47136.
  5. Choi JC, et al. 2007. J. Immunol. 178:1598.
  6. Tjon AS, et al. 2014. J Immunol. 192:5625. PubMed
Product Citations
  1. Tjon A, et al. 2014. J Immunol. 192:5625. PubMed
  2. Kulling PM, et al. 2018. PLoS One. 13:e0193429. PubMed
  3. Ferrando-Martinez S, et al. 2018. J Clin Invest. 128:2089. PubMed
  4. Dela Peña–Ponce MG, et al. 2017. Front Pediatr. 5:15. PubMed
  5. Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed
  6. Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
RRID
AB_314718 (BioLegend Cat. No. 308504)

Antigen Details

Structure
Ig superfamily, class II cytokine receptor family, associates with CDw119, 65 kD
Distribution

All cells, except erythrocytes, at low levels

Function
Transducing signals
Ligand/Receptor
IFN-γ
Biology Area
Immunology
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Basler C, et al. 2002. Int. Rev. Immunol. 21:305.
2. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835.

Gene ID
3459 View all products for this Gene ID
UniProt
View information about IFN-gamma R beta chain on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 08/17/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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