PE anti-human HLA-A,B,C Antibody

Pricing & Availability
W6/32 (See other available formats)
Regulatory Status
Other Names
Major Histocompatibility Class I, MHC class I
Mouse IgG2a, κ
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Product Citations
Human peripheral blood lymphocytes stained with W6/32 PE
  • W632_PE_072607
    Human peripheral blood lymphocytes stained with W6/32 PE
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311405 25 tests £68
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311406 100 tests £148
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MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells. They play an important role in cell-mediated immune responses and tumor surveillance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Cat, Cow, Chimpanzee
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone W6/32 recognizes residues in the N terminus of the human ß2-microglobulin molecule21.

Additional reported applications (for the relevant formats) include: immunoprecipitaton2, Western blotting (non-reducing)3, immunohistochemical staining of acetone-fixed frozen tissue sections4,5, blocking6,7, inhibition of NK cell-mediated lysis10, and activation8,9. Clone W6/32 has been reported not to be suitable for immunohistochemistry on paraffin sections17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 311428) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Darrow TL, et al. 1989. J. Immunol. 142:3329.
  2. Stern P, et al. 1987. J. Immunol. 138:1088.
  3. Tran TM, et al. 2001. Immunogenetics 53:440.
  4. Barbatis C, et al. 1981. Gut 22:985.
  5. Ayyoub M, et al. 2004. Cancer Immunity 4:7.
  6. DeFelice M, et al. 1990. Cell. Immunol. 126:420.
  7. Fayen J, et al. 1998. Int. Immunol. 10:1347.
  8. Turco MC, et al. 1988. J. Immunol. 141:2275.
  9. Geppert TD, et al. 1989. J. Immunol. 142:3763.
  10. Wooden SL, et al. 2005. J. Immunol. 175:1383.
  11. Nagano M, et al. 2007. Blood 110:151.
  12. McLoughlin RM,et al.2008. J. Immunol. 181:1323. PubMed
  13. Takahara M, et al.2008. J. Leukoc. Biol. 83:742. PubMed
  14. Lunemann A, et al.2008. J. Immunol. 181:6170. PubMed
  15. Laing BJ, et al. 2010. J. Thorac Cardiovasc Surg. 139:1402. PubMed
  16. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  17. Vambutas A, et al. 2000. Clin. Diagn. Lab. Immun. 7:79.
  18. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (epitope)
  19. Crivello P, et al. 2013. Hum Immunol. 22:100. PubMed
  20. Jung Y, et al. 2015. Mol Cancer Res. 13:197. PubMed
  21. Shields MJ. Ribaudo RK. 1998. Tissue Antigens. 51(5):567-70. (epitope)
Product Citations
  1. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
  2. Meyer S, et al. 2023. Cell Rep. 42:111995. PubMed
  3. Vidard L, et al. 2023. Immun Inflamm Dis. 11:e749. PubMed
  4. Shen JZ, et al. 2022. Mol Cell. 82:1123. PubMed
  5. Hiatt JB, et al. 2022. Clin Cancer Res. 28:4551. PubMed
  6. Zhang X, et al. 2022. Cell Rep. 40:111363. PubMed
  7. Chimienti R, et al. 2022. Cell Rep. 40:111423. PubMed
  8. Fox DB, et al. 2023. NPJ Precis Oncol. 7:25. PubMed
  9. Feola S, et al. 2021. ACS Nano. 15:15992. PubMed
  10. Oshchepkova A, et al. 2019. Micromachines (Basel). 0.9375. PubMed
  11. Meissner T, et al. 2012. Biochem Biophys Res Commun. 418:786. PubMed
  12. Mayer RL, et al. 2022. Nat Commun. 13:6075. PubMed
  13. Mitchell K, et al. 2018. J Exp Med. 215:1709. PubMed
  14. Izumi K, et al. 2021. Immunohorizons. 5:687. PubMed
  15. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  16. Blank A, et al. 2021. J Pathol. 253:160. PubMed
  17. Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
  18. Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed
  19. Zhang J, et al. 2022. Int J Mol Sci. 23:. PubMed
  20. Zhu XG, et al. 2020. Cell Metabolism. 33(1):211-221.e6. PubMed
  21. Skora A, et al. 2015. Proc Natl Acad Sci U S A. 112: 9967-9972. PubMed
  22. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  23. Herbring V, et al. 2016. Sci Rep. 6:36907. PubMed
  24. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  25. Blees A, et al. 2015. Sci Rep. 5:17341. PubMed
  26. Nazari-Shafti TZ, et al. 2020. Biomolecules. 10:00. PubMed
  27. Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed
  28. Hu W, et al. 2012. Asian J Androl. 14:695. PubMed
  29. Saluzzo S, et al. 2022. STAR Protoc. 3:101470. PubMed
  30. Arakawa A, et al. 2021. J Immunol. 207:2235. PubMed
  31. Singh MV, et al. 2020. J Leukoc Biol. 109:807. PubMed
  32. Salzberger W, et al. 2015. PLoS One. 10: 0145324. PubMed
  33. Vantourout P, et al. 2014. Sci Transl Med. 6:231. PubMed
  34. Palmer CD, et al. 2022. Nat Med. 28:1619. PubMed
  35. Brohl AS, et al. 2021. Cell Rep. 37:110047. PubMed
  36. Chida K, et al. 2021. Biol Pharm Bull. 44:1551. PubMed
  37. Miller MS, et al. 2019. J Biol Chem. 294:19322. PubMed
  38. Martufi M, et al. 2019. CRISPR J. 2:31. PubMed
  39. Iraqi M, et al. 2020. Front Cell Infect Microbiol. 10:48. PubMed
  40. Lee PC, et al. 2022. J Clin Invest. 132:. PubMed
  41. Strijker JGM, et al. 2021. J Pers Med. 11:. PubMed
  42. Grossmann N, et al. 2014. Nat Commun. 5:5419. PubMed
  43. Merlo A, et al. 2010. J Immunol. 184:5895. PubMed
  44. Javitt A, et al. 2019. Front Immunol. 10:141. PubMed
  45. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  46. Liang YH, et al. 2021. J Biomed Sci. 28:75. PubMed
  47. Kenty JH, et al. 2021. STAR Protocols. 2(3):100675. PubMed
  48. Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
AB_314874 (BioLegend Cat. No. 311405)
AB_314875 (BioLegend Cat. No. 311406)

Antigen Details

Ig superfamily

All nucleated cells

Antigen presentation
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Barclay AN, et al. Eds. 1993. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
3105 View all products for this Gene ID
View information about HLA-A on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 11/20/2014

For Research Use Only. Not for diagnostic or therapeutic use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


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