Loading...

PE anti-human CX3CR1 Antibody

Pricing & Availability
Clone
2A9-1 (See other available formats)
Regulatory Status
RUO
Other Names
V28, GPR13, Chemokine C-X3-C receptor 1
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
2A9-1_PE_050109.jpg
Human peripheral blood lymphocytes stained with CD3 APC (UCHT1) and 2A9-1 PE
  • 2A9-1_PE_050109.jpg
    Human peripheral blood lymphocytes stained with CD3 APC (UCHT1) and 2A9-1 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
341603 25 tests £89
Check Availability


Need larger quantities of this item?
Request Bulk Quote
341604 100 tests £208
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediatesfirm cell adhesion and migration.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CX3CR1-EGFP fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Nishimura M, et al. 2002. J. Immunol. 168:6173.
  2. Nanki T, et al. 2002. Arthritis Rheum. 46:2878.
  3. Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837.
  4. Beziat V, et al. 2011. J. Immunol. 186:6753. PubMed.
Product Citations
  1. Cannon J, et al. 2014. Physiol Rep. 2:12177. PubMed
  2. Weiskopf D, et al. 2015. Proc Natl Acad Sci U S A. 112: E4256-E4263. PubMed
  3. Morandi F, et al. 2014. J Immunol. 192:2634. PubMed
  4. Lewis SA, et al. 2021. Front Immunol. 12:724015. PubMed
  5. Izmirly AM, et al. 2022. PLoS Pathog. 18:e1009903. PubMed
  6. Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
  7. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
  8. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  9. Guttikonda SR, et al. 2021. Nat Neurosci. 24:343. PubMed
  10. Agace W, et al. 2014. J Leukoc Biol. 95:531. PubMed
  11. Leers M,et al. 2017. Cytometry A.. 10.1002/cyto.a.23263. PubMed
  12. Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed
  13. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
RRID
AB_1595552 (BioLegend Cat. No. 341603)
AB_1595456 (BioLegend Cat. No. 341604)

Antigen Details

Structure
G-protein-coupled seven transmembrane receptor
Distribution

NK cells, T cell subset, monocytes/macrophages, dendritic cells

Function
cell adhesion and migration
Ligand/Receptor
CX3CL1 (Fractalkine, FKN, neurotactin)
Cell Type
Dendritic cells, Macrophages, Monocytes, NK cells, T cells
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family
Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Imai T, et al. 1997. Cell. 91:521.
2. Fong AM, et al. 1998. J. Exp. Med. 188:1413.
3. Auffray C, et al. 2009. J. Exp. Med. 206:595.

Gene ID
1524 View all products for this Gene ID
UniProt
View information about CX3CR1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE/Cyanine7 anti-human CX3CR1

  • Purified anti-human CX3CR1

  • PE anti-human CX3CR1

  • FITC anti-human CX3CR1

  • Alexa Fluor® 647 anti-human CX3CR1

  • APC anti-human CX3CR1

  • PerCP/Cyanine5.5 anti-human CX3CR1

  • APC/Cyanine7 anti-human CX3CR1

  • Biotin anti-human CX3CR1

  • Brilliant Violet 510™ anti-human CX3CR1

  • Brilliant Violet 421™ anti-human CX3CR1

  • PE/Dazzle™ 594 anti-human CX3CR1

  • Brilliant Violet 650™ anti-human CX3CR1

  • Brilliant Violet 785™ anti-human CX3CR1

  • Brilliant Violet 711™ anti-human CX3CR1

  • APC/Fire™ 750 anti-human CX3CR1
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account