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- Clone
- MKT5.1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LIR-3, HL9
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood monocytes were stained with MKT5.1 PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram)
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 337703 | 25 tests | £100 | ||||
| 337704 | 100 tests | £217 | ||||
CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). CD85a is the 110kD member, known as ILT5, LIR-3, or HL9. ILT5 structurally has four extracellular Ig domains and 4 ITIMs in its cytoplasmic tail that provide inhibitory signals. ILT5 is found on the surface of Monocytes/macrophages, granulocytes, NK cells and T-cell subset. ILT5 provides inhibitory signals through interaction with MHC class I molecules.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Inui M, et al. 2015. Int Immunol. PubMed
- Product Citations
-
- RRID
-
AB_1279285 (BioLegend Cat. No. 337703)
AB_2136536 (BioLegend Cat. No. 337704)
Antigen Details
- Structure
- ILT/LIR family, Ig superfamily, 110 kD
- Distribution
-
Monocytes/macrophages, granulocytes, NK cells and T-cell subset
- Ligand/Receptor
- MHC class I
- Cell Type
- Granulocytes, Macrophages, Monocytes, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
2. Sloane DE, et al. 2004. Blood 104:2832
3. Colonna M, et al. 1998. J. Immunol. 160:3096 - Gene ID
- 11025 View all products for this Gene ID
- UniProt
- View information about CD85a on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD85 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD85a (ILT5) | MKT5.1 | FC |
| APC anti-human CD85a (ILT5) | MKT5.1 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD85a (ILT5)
Human peripheral blood monocytes were stained with MKT5.1 PE... -
APC anti-human CD85a (ILT5)
Human peripheral blood monocytes were stained with MKT5.1 AP...
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