PE anti-human CD80 Antibody

Pricing & Availability
2D10 (See other available formats)
Regulatory Status
VI CD80.1
Other Names
B7-1, B7, BB1
Mouse IgG1, κ
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Product Citations
Human B-cell Burkitt's lymphoma cell line Daudi stained with 2D10 PE
  • 2D10_PE_070507
    Human B-cell Burkitt's lymphoma cell line Daudi stained with 2D10 PE
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305207 25 tests £68
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305208 100 tests £145
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CD80, also known as B7-1, B7, and BB1, is a 60 kD single chain type I glycoprotein belonging to the immunoglobulin superfamily. CD80 is expressed on activated B and T cells, macrophages, and dendritic cells. CD80 binds to CD28 and CD152 (CTLA-4). Along with CD86, CD80 plays a critical role in regulation of T cell activation. The interaction of CD80 with CD28 provides a potent costimulatory signal for T cell activation through the CD3 complex, while its interaction with CTLA-4 provides an inhibitory signal for T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of T cell activation, immunohistochemical staining of acetone-fixed frozen tissue sections2, immunoprecipitation, and Western blotting3. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305245 & 305246).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Battifora M. 1998. J. Clin. Endocr. Metab. 83:4130. (IHC)
  3. Van der Merwe PA, et al. 1997. J. Exp. Med. 185:3. (WB)
  4. Jayakumar A, et al. 2008. Infect. Immun. 76:2138. PubMed
  5. Schubert DA, et al. 2012. J. Exp Med. 209:335. PubMed
  6. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations
  1. Werz O, et al. 2018. Nat Commun. 9:59. PubMed
  2. Lathrop S, et al. 2015. Infect Immun . 83: 2661 - 2671. PubMed
  3. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  4. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  5. Sugawara E, et al. 2020. Autophagy. 1.323611111. PubMed
  6. Rosskopf S, et al. 2016. Sci Rep. 6:31580. PubMed
  7. Hou ZH, et al. 2020. Am J Physiol Cell Physiol. 318:C649. PubMed
  8. Santiago K, et al. 2016. Pathog Dis. 74: ftw081. PubMed
  9. Du X, et al. 2018. Cell Res. 28:416. PubMed
  10. Herath S, et al. 2014. PLoS One. 9:88327. PubMed
  11. Wang D, et al. 2016. J Cell Sci. 129: 2261 - 2272. PubMed
  12. Guo Z, et al. 2022. NPJ Precis Oncol. 6:34. PubMed
  13. Zhang S, et al. 2020. Front Immunol. 1.757638889. PubMed
  14. Jutz S, et al. 2016. J Immunol Methods. 430:10-20. PubMed
  15. Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed
  16. Lewis SA, et al. 2021. Front Immunol. 12:724015. PubMed
  17. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  18. Ganesan R, et al. 2020. FASEB J. 34:15888. PubMed
  19. Zhang L, et al. 2011. Blood. 117:6184. PubMed
  20. Blaszczak AM, et al. 2019. J Diabetes Res. 2019:8124563. PubMed
  21. Serhan J 2012. Blood. 120:e60. PubMed
  22. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  23. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  24. Luque-Martin R, et al. 2021. J Immunol. 207:555. PubMed
  25. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
  26. Kubala MH, et al. 2018. Cell Rep. 25:2177. PubMed
  27. Gärtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed
  28. Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed
  29. Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed
  30. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
  31. Morell M, et al. 2022. EBioMedicine. 76:103808. PubMed
  32. Trabanelli S, et al. 2014. J Immunol. 192:1231. PubMed
AB_314503 (BioLegend Cat. No. 305207)
AB_314504 (BioLegend Cat. No. 305208)

Antigen Details

Ig superfamily, type I transmembrane glycoprotein, 60 kD

Activated B cells and T cells, macrophages, and dendritic cells

T cell costimulation
CD28, CD152 (CTLA-4)
Cell Type
B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Freeman G, et al. 1991. J. Exp. Med. 174:625.
2. Linsley P, et al. 1996. Immunity 4:535.
3. Linsley P, et al. 1991. J. Exp. Med. 174:561.

Gene ID
941 View all products for this Gene ID
View information about CD80 on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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