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PE anti-human CD63 Antibody

Pricing & Availability
Clone
H5C6 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
LIMP, LAMP-3, Melanoma-associated antigen (ME491), Pltgp40, gp55
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
H5C6_PE_090111
Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • H5C6_PE_090111
    Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
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353003 25 tests £78
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353004 100 tests £194
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Description

CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
T cell line HPB-ALL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence2, and immunoprecipitation1.

Application References

(PubMed link indicates BioLegend citation)
  1. Hildreth JE, et al. 1991. Blood 77:121. (IP, WB)
  2. Beatty WL, et al. 2006. J. Cell Sci. 119:350. (IF)
Product Citations
  1. Margaroli C, et al. 2021. Cell Reports Medicine. 2(4):100239. PubMed
  2. Palkowitz AL, et al. 2021. Adv Healthc Mater. 10:e2100132. PubMed
  3. Mastoridis S, et al. 2021. Am J Transplant. 21:2387. PubMed
  4. Sung SE, et al. 2021. Int J Mol Sci. 22:. PubMed
  5. Khan K, et al. 2022. J Tissue Eng Regen Med. 16:110. PubMed
  6. Salybekov AA, et al. 2021. Front Cardiovasc Med. 8:754254. PubMed
  7. Nazari-Shafti TZ, et al. 2020. Biomolecules. 10:00. PubMed
  8. Balbi C, et al. 2021. EBioMedicine. 67:103369. PubMed
  9. Schanin J, et al. 2022. Commun Biol. 5:1226. PubMed
  10. Ponath V, et al. 2021. Int J Mol Sci. 22:. PubMed
  11. DeWeirdt PC, et al. 2020. Nat Biotechnol. . PubMed
  12. Liu JF, et al. 2022. Biomed Res Int. 2022:9453270. PubMed
  13. Mastoridis S, et al. 2018. Front Immunol. 1.474305556. PubMed
  14. Plum T, et al. 2020. Immunity. 52(2):404-416. PubMed
  15. Ding L, et al. 2018. Carcinogenesis. 39:397. PubMed
RRID
AB_10896786 (BioLegend Cat. No. 353003)
AB_10897809 (BioLegend Cat. No. 353004)

Antigen Details

Structure
Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Distribution

Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle

Function
Platelet activation
Interaction
CD9, CD81, VLA-3, and VLA-6
Cell Type
B cells, Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Azorsa DO, et al. 1991. Blood 78:280.
  2. Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford University Press New York.
  3. Hildreth JE, et al. 1991. Blood 77:121.
  4. Anzai N, et al. 2002. Blood 99:4413.
Gene ID
967 View all products for this Gene ID
UniProt
View information about CD63 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 08/25/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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