PE anti-human CD49c (integrin α3) Antibody

Pricing & Availability
Clone
ASC-1 (See other available formats)
Workshop
VI A002
Other Names
α3 integrin, integrin α3 chain, VLA-3 α chain, VLA3a, ITGA3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
ASC-1_PE_081409
Human cervical cancer cell line HeLa cells stained with ASC-1 PE
  • ASC-1_PE_081409
    Human cervical cancer cell line HeLa cells stained with ASC-1 PE
See PE spectral data
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343803 25 tests £81
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Description

CD49c is a 150 kD α integrin chain known as α3 integrin or VLA-3 α chain. It is a type I transmembrane glycoprotein which is proteolytically cleaved into two disulfide linked fragments of 125 kD and 30 kD. CD49c forms a heterodimer with integrin β1 (α3β1, CD49c/CD29, VLA-3) and is expressed by many types of adhesion cells, such as endothelial cells, epithelial cells, and dermal fibroblasts. Weak expression has been reported on leukocytes. VLA-3 plays a role in cell-cell and cell-matrix adhesion through binding Kalinin, collagen, laminin-1, laminin-5, entactin, and fibronectin.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human squamous cell carcinoma cell line SCC-9
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunoprecipitation4, Western blotting5, and immunohistochemical staining of frozen tissue sections2.

Application References

(PubMed link indicates BioLegend citation)
  1. Pattaramalai S, et al. 1996. Exp. Cell. Res. 222:281.
  2. Skubitz AP, et al. 1996. Am. J. Pathol. 148:1445. (IHC)
  3. Skubitz AP, et al. 1998. FEBS Lett. 426:386.
  4. Calzada MJ, et al. 2004. Circ. Res. 94:462. (IP)
  5. Zhang X, et al. 2003. Surgery. 133:429. (WB)
Product Citations
  1. Shimaya M, et al. 2010. Osteoarthritis Cartilage. 18:1300. PubMed
  2. Kamel A, et al. 2016. J Egypt Natl Canc Inst. 28:31-37. PubMed
Publication Library
RRID
AB_1731941 (BioLegend Cat. No. 343803)

Antigen Details

Structure
150 kD type I transmembrane glycoprotein, integrin family, associate with integrin β1(CD29)
Distribution
Endothelial cells, epithelial cells, dermal fibroblast
Function
adhesion
Ligand/Receptor
Kalinin, collagen, laminin-1, laminin-5 and entactin, fibronectin
Cell Type
Endothelial cells, Epithelial cells, Fibroblasts
Biology Area
Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. John Wiley & Sons Inc.

Gene ID
3675 View all products for this Gene ID
UniProt
View information about CD49c on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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