PE anti-human CD44 isoform 9 (CD44v9) Antibody

Pricing & Availability
Clone
RV3 (See other available formats)
Other Names
CD44 variant 9, CD44 variant isoform 9
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
RV3_PE_CD44v9_Antibody_071519_updated.png
PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD44 isoform 9 (CD44v9) (clone RV3) PE (filled histogram) or Rat IgG2a, κ isotype control PE (open histogram).
  • RV3_PE_CD44v9_Antibody_071519_updated.png
    PHA-stimulated (3 days) human peripheral blood lymphocytes were stained with CD44 isoform 9 (CD44v9) (clone RV3) PE (filled histogram) or Rat IgG2a, κ isotype control PE (open histogram).
See PE spectral data
Cat # Size Price Quantity Avail. Save
394403 25 tests £64
Check Availability


Need larger quantities of this item?
Request Bulk Quote
394404 100 tests £155
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD44 isoform 9, also known as CD44v9, is a CD44 isofom consisting of exons 1-5, 13 (v9) and 15 to 19, product of alternative splicing. CD44v9 is expressed by cancer stem cells, epithelial-type carcinomas, subsets of monocytes and subsets of lymphocytes; have a role in tumor initiation, maintenance and metastasis of cancer cells. CD44v9 interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells, and is a potential predictive marker for recurrence of some cancers and a potential therapeutic target.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD44v8-10 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections3.

Application References

(PubMed link indicates BioLegend citation)
  1. Kakehashi, A., et al. 2016. Cancer Sci. 107: 609. (IHC-P)
  2. Aso T, et al. 2015. PLoS One. 10: e0116596. (IHC-P)
  3. Yoshikawa M, et al. 2013. Cancer Res. 73: 1855. (IHC-F)
  4. Ishimoto T, et al. 2011. Cancer Cell. 387-400. (IHC-P)

Antigen Details

Distribution

Cancer stem cells, epithelial-type carcinomas, subset of monocytes, subset of lymphocytes.

Function
Role in tumor initiation and maintenance and metastasis of cancer cells, Interacts with the glutamate-cystine transporter xCT, increasing reduced glutathione and contributing to the ROS resistance of cancer cells.
Cell Type
Epithelial cells, Lymphocytes, Monocytes, Neutrophils
Biology Area
Cancer Biomarkers, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Hagiwara M, et al. 2018. BMC Cancer. 18:113
  2. Matsumoto T, et al. 2017. Oncogenesis. 6:397
  3. Bertaux-Skeirik N, et al. 2017. J. Pathol. 242:463
  4. Kodama H, et al. 2017. Br. J. Cancer. 116:186
Gene ID
960 View all products for this Gene ID
UniProt
View information about CD44v9 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/03/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account