PE anti-human CD314 (NKG2D) Antibody

Pricing & Availability
Clone
1D11 (See other available formats)
Regulatory Status
RUO
Other Names
NKG2D
Isotype
Mouse IgG1, κ
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Product Citations
publications
1D11_PE_022006
Human peripheral blood lymphocytes were stained with CD314 (clone 1D11) PE and CD8 PE/Cy5.
  • 1D11_PE_022006
    Human peripheral blood lymphocytes were stained with CD314 (clone 1D11) PE and CD8 PE/Cy5.
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320805 25 tests £84
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320806 100 tests £184
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Description

CD314 is a homodimeric C-type lectin-like protein also known as NKG2D. It is expressed on NK cells, CD8+ T cells, γ/δ T cells, and in vitro induced LAK cells. Several molecules have been identified as the ligands for NKG2D, including MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs). NKG2D has no intrinsic signaling capacity, but attains this by non-covalent association with DAP10 or DAP12 adaptors. In addition to being a primary activation receptor on NK cells, NKG2D is also a costimulatory receptor for TCR-mediated T cell proliferation and cytokine production. The interaction of NKG2D with its ligands plays a role in the immune surveillance against pathogen and tumor cells, and in the pathogenesis of autoimmune diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 1D11 antibody blocks MICA binding to T cells, induces redirected lysis, and costimulates T cells activation and proliferation. Additional reported (for the relevant formats) applications include: immunoprecipitation1,2, blocking of ligand binding, induction of redirected cell lysis, and costimulation of T cells proliferation2-7. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 320814) with endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered.

Application References

(PubMed link indicates BioLegend citation)
  1. Wu J, et al. 1999. Science 285:730.
  2. Wu J, et al. 2000. J. Exp. Med. 192:1059.
  3. Groh V, et al. 2001. Nature Immunol. 2:255.
  4. Wu J, et al. 2002. J. Immunol. 169:1236.
  5. Roberts A, et al. 2001. J. Immunol. 167:5527.
  6. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.
  7. Kraetzel K et al. 2008. Eur. Respir. J. 32:563. PubMed
  8. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  9. Watanbe M, et al. 2014. Int Immunol. PubMed
Product Citations
  1. Firouzi J, et al. 2023. Cell J. 25:92. PubMed
  2. Dorsch M, et al. 2020. Eur J Immunol. 50:656. PubMed
  3. Höfle J, et al. 2022. EMBO Rep. 23:e54133. PubMed
  4. Gerace D, et al. 2023. Cell Rep Med. 4:100879. PubMed
  5. Liu R, et al. 2022. Aging Dis. 13:1576. PubMed
  6. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  7. Lee Y, et al. 2020. J Innate Immun. 1:. PubMed
  8. Myers JA, et al. 2022. JCI Insight. :. PubMed
  9. Whitman E, Barber A 2015. Mol Immunol. 63:268. PubMed
  10. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  11. Karampatzakis A, et al. 2021. Front Immunol. 12:641521. PubMed
  12. Zhou J, et al. 2015. J Immunol. 194:4688. PubMed
  13. Mohan S, et al. 2009. Haematologica. 94:1407. PubMed
  14. ça T, et al. 2010. Blood. 115:2407. PubMed
  15. Sweeney EE, et al. 2020. Nano Res. 13:736. PubMed
  16. Tello-Lafoz M, et al. 2021. Immunity. 54(5):1037-1054.e7. PubMed
  17. Hejazi M, et al. 2022. Front Immunol. 12:798087. PubMed
  18. Wolpert F, et al. 2015. PLoS One. 10: e0139603. PubMed
  19. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
  20. Sharma N,et al. 2017. J Immunol. 10.4049/jimmunol.1700647. PubMed
  21. Samarani S, et al. 2020. Mediators Inflamm. 2020:6401969. PubMed
  22. Chu P, et al. 2015. PLoS One. 10:125664. PubMed
  23. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
  24. Vidard L, et al. 2019. J Immunol. 203:676. PubMed
  25. Chu P, et al. 2017. PLoS One. 12(6):e0179096. PubMed
  26. Li M, et al. 2020. Virol Sin. 35:588. PubMed
  27. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
RRID
AB_492961 (BioLegend Cat. No. 320805)
AB_492960 (BioLegend Cat. No. 320806)

Antigen Details

Structure
C-type lectin
Distribution

NK cells, γ/δ T cells, CD8+ T cells

Function
Cytolytic killing of target cells expressing NKG2D ligands, costimulation of NK cells and T cells
Ligand/Receptor
MICA, MICB, UL16-binding proteins (ULBPs)
Cell Type
NK cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Raulet DH. 2003. Nat. Rev. Immunol. 3:781.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Jamieson AM, et al. 2002. Immunity 17:19.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.
7. Maasho K, et al. 2005. J. Immunol. 174:4480.
8. Groh V, et al. 2003. Proc. Natl. Acad. Sci. USA 100:9452.

Gene ID
22914 View all products for this Gene ID
UniProt
View information about CD314 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
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For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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