PE anti-human CD27 Recombinant Antibody

Pricing & Availability
Clone
QA17A18 (See other available formats)
Other Names
S152, T14, TNFRSF7
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA17A18_PE_CD27_Antibody_062718
Human peripheral blood lymphocytes were stained with CD3 APC and PE anti-human CD27 (clone QA17A18) (left) or PE mouse IgG1, κ isotype control (right).
  • QA17A18_PE_CD27_Antibody_062718
    Human peripheral blood lymphocytes were stained with CD3 APC and PE anti-human CD27 (clone QA17A18) (left) or PE mouse IgG1, κ isotype control (right).
See PE spectral data
Cat # Size Price Quantity Avail. Save
393211 25 tests £64
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393212 100 tests £185
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Description

CD27 is a 50-55 kD type I membrane protein also known as S152 and T14. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in costimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone QA17A18 does not block clones 0323, M-T271, or LG.3A10, indicating a unique epitope.

Antigen Details

Structure
TNF-R superfamily, type I transmembrane glycoprotein.
Distribution

Medullary thymocytes, T and B cell subsets, NK cells.

Ligand/Receptor
CD70
Cell Type
B cells, NK cells, T cells, Thymocytes
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, TCRs
Antigen References
  1. Knapp W, et al. 1989. Immunol. Today 10:253-8
  2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
  3. Hintzen R, et al. 1994. Immunol. Today 15:307.
  4. Agematsu K, et al. 1995. J. Immunol. 154:3627.
Gene ID
939 View all products for this Gene ID
UniProt
View information about CD27 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 06/27/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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