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PE anti-human CD262 (DR5, TRAIL-R2) Antibody

Pricing & Availability
Clone
DJR2-4 (7-8) (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
TRAIL-R2, KILLER, TRICK2, TNFRSF10B, Ly98, CD262
Isotype
Mouse IgG1, κ
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Product Citations
publications
DJR2-4_PE_072007
Human DR5 transfected cells stained with DJR2-4 PE
  • DJR2-4_PE_072007
    Human DR5 transfected cells stained with DJR2-4 PE
Compare all formats See PE spectral data
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307405 25 tests £92
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307406 100 tests £208
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Description

DR5 is 55 kD member 10B of the TNF receptor superfamily (TNFRSF10B), also known as TRAIL-R2, TRICK2, KILLER, and CD262. It binds the cytotoxic ligand TRAIL and induces apoptosis. The DR5 receptor is broadly expressed on a variety of normal tissues and many tumors. DR5 expression has been reported to be upregulated in human cells by interferon-α, 2-methoxyestradiol, and paclitaxel, and downregulated by adenoviral E3 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of DR5-human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: The DJR2-4 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR5/TRAIL-R2 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 307406) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 307404) or biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Uno K, et al. 2003. Blood 101:3658.
  2. Sato K, et al. 2005. J. Immunol. 174:4025.
  3. Lundgvist A,et al.2006.Cancer Res.14:7317.PubMed
Product Citations
  1. Braun F, et al. 2015. PLoS One. 10:117994. PubMed
  2. Grayson KA, et al. 2021. Mol Cancer Ther. 20:833. PubMed
  3. Jeong D, et al. 2021. eLife. 0.416666666666667. PubMed
  4. Vunnam N, et al. 2017. Biophys J. 10.1016/j.bpj.2017.05.038. PubMed
  5. Wu Y, et al. 2019. Sci Rep. 9:20301. PubMed
  6. Yerbes R, et al. 2022. Cell Death Dis. 13:906. PubMed
  7. Ng W, et al. 2021. Pharm Biol. 59:47:00. PubMed
  8. Bernareggi D, et al. 2022. Nat Commun. 13:1899. PubMed
  9. Grayson KA, et al. 2021. PLoS One. 16:e0246733. PubMed
  10. Magnussen G, et al. 2012. Biochem Biophys Res Commun. 420:516. PubMed
  11. Yao C, et al. 2018. Autophagy. 1.854861111. PubMed
  12. Volpin V, et al. 2020. Cancer Immunol Res. 8:1163. PubMed
  13. Greenlee JD, et al. 2021. eLife. 0.416666666666667. PubMed
  14. Ray JE, et al. 2021. Cancer Biol Ther. 22:554. PubMed
  15. Kolluri KK et al. 2018. eLife. 7 pii: e30224. PubMed
  16. Brin E, et al. 2018. Oncotarget. 9:36914. PubMed
  17. Stolfi C, et al. 2011. Mol Cancer Ther. 1.159027778. PubMed
  18. Ishii Y, et al. 2021. J Biol Chem. 297:101223. PubMed
  19. Choi Y, et al. 2015. Gut. 64: 1303-1313. PubMed
  20. Canestraro M, et al. 2010. Cancer Genet Cytogenet. 199:110. PubMed
RRID
AB_314677 (BioLegend Cat. No. 307405)
AB_2204926 (BioLegend Cat. No. 307406)

Antigen Details

Structure
TNFR superfamily, 55 kD
Distribution

Broadly expressed on normal and tumor cells

Function
Induces apoptosis
Ligand/Receptor
TRAIL
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. MacFarlane M, et al. 1997. J. Biol. Chem. 272:25417.
2. Walczak H, et al. 1997. EMBO J. 16:5386.
3. Shigeno M, et al. 2003. Oncogene 22:1653.
4. LaVallee T, et al. 2003. Cancer Res. 63:468.
5. Nimmanapalli R, et al. 2001. Cancer Res. 61:759

Gene ID
8795 View all products for this Gene ID
UniProt
View information about CD262 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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