PE anti-human CD253 (TRAIL) Antibody

Pricing & Availability
Clone
RIK-2 (See other available formats)
Regulatory Status
RUO
Other Names
Apo-2 ligand, Apo-2L, TNFSF10, CD253
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
RIK-2_PE_072007
Human TRAIL transfected cells stained with RIK-2 PE
  • RIK-2_PE_072007
    Human TRAIL transfected cells stained with RIK-2 PE
See PE spectral data
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308205 25 tests £72.00
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308206 100 tests £171.00
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Description

TRAIL is a 30 kD protein known as TNF-related apoptosis inducing ligand, CD253, TNFSF10, or Apo-2L. It can be expressed as a cell surface (30 kD) and soluble ligand (19-20 kD) produced by megakaryocytes and platelets, monocytes, neutrophils, NK cells and activated T cells. This antigen is also expressed on tumor cells and transformed cell lines. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. This antibody is useful for flow cytometric staining and blocking studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TRAIL-transfected mouse cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking1-4 of TRAIL-induced apoptosis and T cell mediated cytotoxicity. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308214).

Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1999. J. Immunol. 162:2639. (Block)
  2. Uno K, et al. 2003. Blood 101:3658. (Block)
  3. Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
  4. Denny MF, et al. 2007. Blood 110:2907. (Block)
  5. Kemter E, et al. 2011. Xenotransplantation. 19:40. PubMed
Product Citations
  1. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  2. Achard C, et al. 2017. Oncoimmunology. 6:e1261240. PubMed
  3. Kalb M, et al. 2012. J Immunol. 188:1583. PubMed
  4. Choi Y, et al. 2015. Gut. 64: 1303-1313. PubMed
  5. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  6. Volpin V, et al. 2020. Cancer Immunol Res. 8:1163. PubMed
RRID
AB_345291 (BioLegend Cat. No. 308205)
AB_2205825 (BioLegend Cat. No. 308206)

Antigen Details

Structure
TNF ligand superfamily member, approximately 30 kD
Distribution

Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells

Function
Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity, TRAIL ligand of cognate receptors induces NF-κB signaling in a variety of cell types
Ligand/Receptor
DR4 and DR5 cognate receptors, also binds to the decoy receptors DcR1 and DcR2
Cell Type
Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Pitti RM, et al. 1996. J. Biol. Chem. 271:12687.
2. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.
3. Dorothee G, et al. 2002. J. Immunol. 169:809.
4. Crist SA, et al. 2004. Exp. Hematology 32:1073.

Gene ID
8743 View all products for this Gene ID
UniProt
View information about CD253 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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