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PE anti-human CD206 (MMR) Antibody

Pricing & Availability
Clone
15-2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1
Isotype
Mouse IgG1, κ
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Product Citations
publications
15-2_PE_090707
GM-CSF-stimulated (day-3) human monocytes stained with 15-2 PE
  • 15-2_PE_090707
    GM-CSF-stimulated (day-3) human monocytes stained with 15-2 PE
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321105 25 tests £84
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321106 100 tests £176
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Description

Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human mannose receptor
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).

Application References

(PubMed link indicates BioLegend citation)
  1. Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block)
  2. Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block)
  3. Kato M, et al. 2007. J. Immunol. 179:6052. (IF)
Product Citations
  1. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  2. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  3. Shen Y, et al. 2021. Cell Stress Chaperones. 26:937. PubMed
  4. Zhan X, et al. 2014. Biomaterials. 35:10046. PubMed
  5. Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed
  6. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
  7. Zhou WH, et al. 2022. Discov Oncol. 13:67. PubMed
  8. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  9. Dan H, et al. 2020. Mol Oncol. 14:795. PubMed
  10. Jaén RI, et al. 2022. Int J Mol Sci. 23:. PubMed
  11. Rao X, et al. 2022. Cell Death Dis. 13:891. PubMed
  12. Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed
  13. Malekghasemi S, et al. 2020. Cell Biol Int. 44:2031. PubMed
  14. Yang M, et al. 2018. Oncol Lett. 15:3918. PubMed
  15. Zangeneh Z, et al. 2022. Iran J Basic Med Sci. 25:474. PubMed
  16. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  17. Forsberg MH, et al. 2021. Stem Cell Res Ther. 12:459. PubMed
  18. Qin XH, et al. 2019. Langmuir. 35:1882. PubMed
  19. Lv J, et al. 2021. Mol Med Rep. 1. PubMed
  20. Cho JH, et al. 2021. Nat Commun. 12:792. PubMed
  21. Madsen NH, et al. 2021. Pathogens. 10:. PubMed
  22. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  23. Soberanes S et al. 2018. Cell metabolism. 29(2):335-347 . PubMed
  24. Kleinnijenhuis J, et al. 2012. Proc Natl Acad Sci U S A. 109:17537. PubMed
  25. Wilcz-Villega E, et al. 2020. EMBO Mol Med. 12:e10491. PubMed
  26. Georgouli M et al. 2019. Cell. 176(4):757-774 . PubMed
  27. Chen X, et al. 2022. Cell Commun Signal. 20:92. PubMed
RRID
AB_571910 (BioLegend Cat. No. 321105)
AB_571911 (BioLegend Cat. No. 321106)

Antigen Details

Structure
Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD
Distribution

Macrophages, dendritic cells, hepatic and lymphatic endothelial cells

Function
Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand/Receptor
Mannose, fucose, N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303
2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469.
4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934.
5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.

Gene ID
4360 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD206 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD206 (MMR) 15-2 FC,WB,ICC,IHC-F
FITC anti-human CD206 (MMR) 15-2 FC
PE anti-human CD206 (MMR) 15-2 FC
PE/Cyanine5 anti-human CD206 (MMR) 15-2 FC
APC anti-human CD206 (MMR) 15-2 FC
Alexa Fluor® 488 anti-human CD206 (MMR) 15-2 FC
Alexa Fluor® 647 anti-human CD206 (MMR) 15-2 FC
Biotin anti-human CD206 (MMR) 15-2 FC
APC/Cyanine7 anti-human CD206 (MMR) 15-2 FC
PerCP/Cyanine5.5 anti-human CD206 (MMR) 15-2 FC
PE/Cyanine7 anti-human CD206 (MMR) 15-2 FC
Brilliant Violet 421™ anti-human CD206 (MMR) 15-2 FC
Purified anti-human CD206 (MMR) (Maxpar® Ready) 15-2 FC,CyTOF®
Alexa Fluor® 700 anti-human CD206 (MMR) 15-2 FC
PE/Dazzle™ 594 anti-human CD206 (MMR) 15-2 FC
APC/Fire™ 750 anti-human CD206 (MMR) 15-2 FC
Brilliant Violet 711™ anti-human CD206 (MMR) 15-2 FC
Brilliant Violet 510™ anti-human CD206 (MMR) 15-2 FC
Brilliant Violet 605™ anti-human CD206 (MMR) 15-2 FC
Brilliant Violet 785™ anti-human CD206 (MMR) 15-2 FC
TotalSeq™-A0205 anti-human CD206 (MMR) 15-2 PG
TotalSeq™-B0205 anti-human CD206 (MMR) 15-2 PG
TotalSeq™-C0205 anti-human CD206 (MMR) 15-2 PG
Ultra-LEAF™ Purified anti-human CD206 (MMR) 15-2 FC,WB,ICC,IHC-F
Pacific Blue™ anti-human CD206 (MMR) 15-2 FC
PE/Fire™ 700 anti-human CD206 (MMR) 15-2 FC
TotalSeq™-D0205 anti-human CD206 (MMR) 15-2 PG
Spark NIR™ 685 anti-human CD206 (MMR) 15-2 FC
Spark Red™ 718 anti-human CD206 (MMR) (Flexi-Fluor™) 15-2 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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