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PE anti-human CD179b (Ig λ5) Antibody

Pricing & Availability
Clone
HSL11 (See other available formats)
Regulatory Status
RUO
Other Names
Ig Lambda 5, λ5, Ig omega
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HSL11_PE_081210
Human cell lines REH (pro-B, blue), NALM-6 (pre-B, orange) and Ramos (B cell, open) stained with HSL11 PE
  • HSL11_PE_081210
    Human cell lines REH (pro-B, blue), NALM-6 (pre-B, orange) and Ramos (B cell, open) stained with HSL11 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
349803 25 tests £100
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349804 100 tests £217
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Description

CD179b, also known as Ig Lambda 5, λ5, and Ig omega, is a 22 kD, C-like protein member within the Ig superfamily. λ5 associates with CD179a (VpreB) to form a surrogate light (SL) chain that in turn associates with the IgM heavy chain and the CD79a/CD79b heterodimer to form the pre-B cell receptor (PreBCR) complex. The function of CD179a/CD179b is still under investigation, though it has been reported that the deficiency of CD179a and/or CD179b impairs the maturation of the B cell precursors beyond the pre-B cell stage.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and immunohistochemistry of frozen and paraffin-embedded samples2.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsyganezawa K, et al. 1998. Blood 92:4317. (IP)
  2. Kiyokawa N, et al. 2004. Modern Pathol. 17:423. (ICFC, IHC)
RRID
AB_10643420 (BioLegend Cat. No. 349803)
AB_10643421 (BioLegend Cat. No. 349804)

Antigen Details

Structure
Immunoglobine (Ig) V-like protein. Molecular weight 22 kD. Associates with CD179a (VpreB) to form the surrogate light (SL) chain, which binds to the the Ig µ heavy chain and CD79a/CD79b heterodimer complex to form the pre-B cell receptor (preBCR).
Distribution

Pro-B and early Pre-B cells

Cell Type
B cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc.
2. Zhang M, et al. 2004. Cell. Mol. Immunol. 1:89.
3. Hendriks RW and Middendorp S. 2004. Trends Immunol. 25:249.

Gene ID
3543 View all products for this Gene ID
UniProt
View information about CD179b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD179b (Ig λ5)

  • PE anti-human CD179b (Ig λ5)
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