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PE anti-human CD147 Antibody

Pricing & Availability
Clone
HIM6 (See other available formats)
Regulatory Status
RUO
Workshop
VI N-L109
Other Names
Neurothelin, Basigin, EMMPRIN
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HIM6_PE_CD147_Antibody_072718
Human peripheral blood lymphocytes were stained with CD147 (clone HIM6) PE (filled histogram) or Mouse IgG1, κ PE isotype control (open histogram).
  • HIM6_PE_CD147_Antibody_072718
    Human peripheral blood lymphocytes were stained with CD147 (clone HIM6) PE (filled histogram) or Mouse IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
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306211 25 tests £81
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306212 100 tests £180
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Description

CD147, also known as neurothelin or basigin, is a member of the Ig superfamily. It is a 55-65 kD type I transmembrane glycoprotein which is primarily expressed on leukocytes, erythrocytes, platelets, and endothelial cells. CD147 is reported to have a function during embryonal brain development and/or play a role in integrin-mediated adhesion in brain endothelia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Baboon, Capuchin Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PBMCs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of T cell activation2, immunohistochemical staining1,3 of frozen tissue sections and formalin-fixed paraffin-embedded tissue sections, and Western blotting1. The Ultra-LEAF™ Purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 306221 and 306222).

Application References

(PubMed link indicates BioLegend citation)
  1. Menashi S, et al. 2003. Cancer Res. 63:7575. (WB IHC)
  2. Woodhead VE, et al. 2000. Int. Immunol. 12:1051. (Block)
  3. Reimers N, et al. 2004. Clin. Cancer Res. 10:3422. (IHC)
Product Citations
  1. Barrett TJ, et al. 2021. J Thromb Haemost. 19:3139. PubMed
RRID
AB_2750167 (BioLegend Cat. No. 306211)
AB_2750168 (BioLegend Cat. No. 306212)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55-65 kD
Distribution

Leukocytes, erythrocytes, platelets, endothelial cells

Function
Adhesion
Cell Type
Endothelial cells, Erythrocytes, Leukocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Biswas C, et al. 1995. Cancer Res. 55:434.
2. Fadool J, et al. 1993. Dev. Dyn. 196:252.
3. Felzmann T, et al. 1991. J. Clin. Immunol. 11:205.

Gene ID
682 View all products for this Gene ID
UniProt
View information about CD147 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 07/27/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human CD147

  • Purified anti-human CD147

  • Alexa Fluor® 488 anti-human CD147

  • Alexa Fluor® 647 anti-human CD147

  • PE anti-human CD147

  • APC/Fire™ 750 anti-human CD147

  • APC anti-human CD147

  • PE/Cyanine7 anti-human CD147

  • PerCP/Cyanine5.5 anti-human CD147

  • Ultra-LEAF™ Purified anti-human CD147

  • TotalSeq™-C1137 anti-human CD147

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