PE anti-human CD112R (PVRIG) Antibody

Pricing & Availability
Clone
W16216D (See other available formats)
Regulatory Status
RUO
Other Names
Poliovirus receptor-related immunoglobulin domain containing protein, PVRIG, C7orf15, CD112 receptor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16216D_PE_CD112R_Antibody_050520
Jurkat cells were stained with PE anti-human CD112R (PVRIG) (clone W16216D) (filled histogram) or PE rat IgG2a, κ isotype control (open histogram).
  • W16216D_PE_CD112R_Antibody_050520
    Jurkat cells were stained with PE anti-human CD112R (PVRIG) (clone W16216D) (filled histogram) or PE rat IgG2a, κ isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
301503 25 tests £86.00
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301504 100 tests £209.00
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Description

CD112R gene encodes a single transmembrane protein consisting of a single extracellular IgV domain. Its long intracellular domain contains two tyrosine residues, one of which is within an ITIM-like motif. CD112R was initially named PVRIG for the homology observed between its second exon and the variable immunoglobulin domain of the polio virus receptor (PVR/CD155) and polio virus receptor-like (PVRL) genes. CD112R is a cell surface receptor for CD112/Nectin-2, it compete against CD226 in binding to CD112.  Following interaction with CD112, CD112R inhibits T-cell proliferation and the disruption of interaction between CD112R and CD112 enhances T cell response. Published studies suggest that CD112R is a novel co-inhibitory receptor, or a checkpoint molecule,  that suppressed TCR medicated signal.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD112R transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2860765 (BioLegend Cat. No. 301503)
AB_2876585 (BioLegend Cat. No. 301504)

Antigen Details

Structure
IgV domain; ITIM motif
Distribution

T cells, NK cells

Function
Cell surface receptor for CD112, negative regulator of T cell response
Ligand/Receptor
CD112 (Nectin-2)
Cell Type
Leukocytes, Lymphocytes, NK cells, T cells
Biology Area
Adaptive Immunity, Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family
Immune Checkpoint Receptors
Antigen References
  1. Xu F, et al. 2017. Cancer Immunol. Immunother. 66:1367-1375.
  2. Zhu Y, et al. 2016. J. Exp. Med. 213(2):167-76.
Gene ID
79037 View all products for this Gene ID
UniProt
View information about CD112R on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 05/05/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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