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PE anti-human CD106 Antibody

Pricing & Availability
Clone
STA (See other available formats)
Regulatory Status
RUO
Workshop
V A013
Other Names
VCAM-1, INCAM-110
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
1_STA
TNF-a stimulated HUVEC cells stained with STA PE
  • 1_STA
    TNF-a stimulated HUVEC cells stained with STA PE
  • 2_3_Human_LN_CD23_CD106
    Confocal image of human lymph node sample acquired using the IBEX method of highly multiplexed antibody-based imaging: CD23 (yellow) in Cycle 2, CD106 (blue) in Cycle 8. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
  • 3_26_Human_Jejunum_Hoechst_CD106_CD31
    Confocal image of human jejunum sample acquired using the IBEX method of highly multiplexed antibody-based imaging: Hoechst (blue) in Cycle 1, CD106 (yellow) in Cycle 1, and CD31 (red) in Cycle 3. Tissues were prepared using ~1% (vol/vol) formaldehyde and a detergent. Following fixation, samples are immersed in 30% (wt/vol) sucrose for cryoprotection. Images are courtesy of Drs. Andrea J. Radtke and Ronald N. Germain of the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
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305805 25 tests £76
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305806 100 tests £167
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Description

CD106 is a 110 kD single chain type I glycoprotein also known as VCAM-1 and INCAM-110. It is expressed predominantly on activated vascular endothelium but has also been identified on follicular and interfollicular dendritic cells, some macrophages, bone marrow stromal cells, and non-vascular cell populations within joints, kidney, muscle, heart, placenta, and brain. Expression on endothelial cells as well as many other cells is induced by inflammatory stimuli and cytokines. Activated endothelial cells can release soluble forms of CD106 which can be detected in the blood. CD106 binds the integrins CD49d/CD29 (VLA-4) and α4β7 that contribute to leukocyte adhesion, transmigration, and co-stimulation of T cell proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining of acetone-fixed frozen tissue sections, immunoprecipitation2, ELISA2 capture for sCD106, and spatial biology (IBEX)5,6.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Leca G, et al. 1995. J. Immunol. 154:1069. (ELISA IP)
  3. Yen YT, et al. 2006. J. Virol. 80:2648. (IF) PubMed
  4. Dmitrieva NI, et al. 2015. PloS One.10:128870. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Melzer C, et al. 2020. Int J Mol Sci. :21. PubMed
  2. Song K, et al. 2012. Exp Cell Res. 318:1707. PubMed
  3. Godavarthy PS, et al. 2021. Front Cell Dev Biol. 9:675240. PubMed
  4. Kho D, et al. 2017. PLoS One. 10.1371/journal.pone.0180267. PubMed
  5. Wagner B, et al. 2011. J Cell Sci. 124:1644. PubMed
  6. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  7. Jin D, et al. 2011. Regul Pept. 166:21. PubMed
  8. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  9. Lewis D, et al. 2016. Cardiovasc Res. 109: 283 - 293. PubMed
  10. Ucci M, et al. 2019. Oxid Med Cell Longev. 2019:8184656. PubMed
  11. Aomatsu E, et al. 2014. Sci Rep. 4:3652. PubMed
  12. Baldassarre MPA, et al. 2021. Nutrients. 13:. PubMed
RRID
AB_314561 (BioLegend Cat. No. 305805)
AB_314562 (BioLegend Cat. No. 305806)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 110 kD
Distribution

Activated endothelial cells, endothelial progenitors, follicular dendritic cells

Function
Leukocyte adhesion, transmigration, costimulation
Ligand/Receptor
VLA-4 (CD49d/CD29)
Cell Type
Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Carlos T, et al. 1994. Blood 84:2068.
2. Jones E, et al. 1995. Nature 373:539.

Gene ID
7412 View all products for this Gene ID
UniProt
View information about CD106 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Go To Top Version: 2    Revision Date: 04/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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