Pacific Blue™ anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
3 reviews
Product Citations
publications
104_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ 104
  • 104_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ 104
See Pacific Blue™ spectral data
Cat # Size Price Quantity Avail. Save
109819 25 µg £57
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109820 100 µg £131
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Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .
Concentration
0.5 mg/ml
Storage & Handling
The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Kincaid E, et al. 2007. J Immunol. 179:3187. PubMed
  2. Pascal V, et al. 2007. J Immunol. 179:1751. PubMed
  3. Reddy Y 2009. Proc Natl Acad Sci U S A. 106:21689. PubMed
  4. Zlotoff D, et al. 2010. Blood. 115:1897. PubMed
  5. Kratky W, et al. 2011. Proc Natl Acad Sci U S A. 108:17414. PubMed
  6. Silva D, et al. 2011. Diabetes. 60:2102. PubMed
  7. Müller A, et al. 2012. Immunity. 37:147. PubMed
  8. Lin G, et al. 2013. PLoS One. 8:e56014. PubMed
  9. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
  10. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  11. Teijaro J, et al. 2014. Proc Natl Acad Sci U S A. 111:3799. PubMed
  12. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  13. Cohen M, et al. 2014. EMBO J. 33:2906. PubMed
  14. Dewas C, et al. 2015. J Immunol. 194:1372. PubMed
  15. Zhu Y, et al. 2015. Neurobiol Dis. 74:114. PubMed
  16. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  17. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  18. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  19. Scholz F, et al. 2015. J Immunol. 195: 2335-2342. PubMed
  20. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
  21. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  22. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  23. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
  24. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
  25. Astuti Y, et al. 2017. Stem Cell Reports. 8(1):177-190. PubMed
  26. Nagalingam G, et al. 2017. J Immunol. 10.4049/jimmunol.1602069. PubMed
  27. Chung L 2018. Cell host & microbe. 23:203. PubMed
  28. Yamamoto R 2018. Cell stem cell. 22:600. PubMed
  29. Gaya M 2018. Cell. 172:517. PubMed
  30. Vasamsetti SB 2018. Immunity. 49:93. PubMed
  31. Lu Y 2018. Cancer cell. 33:1048. PubMed
  32. Rodda LB 2018. Immunity. 48:1014. PubMed
  33. Chhatbar C 2018. Cell reports. 25:118. PubMed
  34. Turner JS, et al. 2018. Cell Rep. 25:1395. PubMed
  35. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
Publication Library
RRID
AB_492873 (BioLegend Cat. No. 109819)
AB_492872 (BioLegend Cat. No. 109820)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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