- dG9 (See other available formats)
- Regulatory Status
- Other Names
- PRF1, P1, PFP, HPLH2
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- Purified granules from the human lymphoma cell line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Clone dG9 primarily recognizes perforin associated with cytotoxic granules9. Additional reported applications (for the relevant formats) include: immunoprecipitation, intracellular flow cytometric analysis and immunofluorescence microscopy5,7, and immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections1,4.
Does not cross-react with mouse1.
(PubMed link indicates BioLegend citation)
- Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
- Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
- Watanabe N, et al. 1997. Blood 90:3662.
- Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
- Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
- Chen H, et al. 2005. J. Immunol. 175:591.
- Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
- Wood SM, et al. 2009. Blood 114:4117. PubMed
- Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.
- Product Citations
AB_10895768 (BioLegend Cat. No. 308117)
AB_10899565 (BioLegend Cat. No. 308118)
- 70 kD
CTL, NK (cytoplasmic granules)
- Mediates targeted cell lysis
- Cell Type
- NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Antigen References
1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
- Gene ID
- 5551 View all products for this Gene ID
- View information about Perforin on UniProt.org
Related Pages & Pathways
Other FormatsView All Perforin Reagents Request Custom Conjugation
|FITC anti-human Perforin||dG9||ICFC|
|PE anti-human Perforin||dG9||ICFC|
|Purified anti-human Perforin||dG9||ICFC,IP,ICC,IHC|
|Alexa Fluor® 488 anti-human Perforin||dG9||ICFC|
|Alexa Fluor® 647 anti-human Perforin||dG9||ICFC|
|APC anti-human Perforin||dG9||ICFC|
|Pacific Blue™ anti-human Perforin||dG9||ICFC|
|PerCP/Cyanine5.5 anti-human Perforin||dG9||ICFC|
|Brilliant Violet 510™ anti-human Perforin||dG9||ICFC|
|Brilliant Violet 421™ anti-human Perforin||dG9||ICFC|
|Alexa Fluor® 594 anti-human Perforin||dG9||ICC|
|APC/Cyanine7 anti-human Perforin||dG9||ICFC|
|PE/Cyanine7 anti-human Perforin||dG9||ICFC|
|Brilliant Violet 711™ anti-human Perforin||dG9||ICFC|
|PE/Dazzle™ 594 anti-human Perforin||dG9||ICFC|
|TotalSeq™-B0044 anti-human Perforin||dG9||ICPG|
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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FITC anti-human Perforin
PE anti-human Perforin
Purified anti-human Perforin
Alexa Fluor® 488 anti-human Perforin
Alexa Fluor® 647 anti-human Perforin
APC anti-human Perforin
Pacific Blue™ anti-human Perforin
PerCP/Cyanine5.5 anti-human Perforin
Brilliant Violet 510™ anti-human Perforin
Brilliant Violet 421™ anti-human Perforin
Alexa Fluor® 594 anti-human Perforin
APC/Cyanine7 anti-human Perforin
PE/Cyanine7 anti-human Perforin
Brilliant Violet 711™ anti-human Perforin
PE/Dazzle™ 594 anti-human Perforin
TotalSeq™-B0044 anti-human Perforin