Pacific Blue™ anti-human Lineage Cocktail (CD3, CD14, CD16, CD19, CD20, CD56)

Pricing & Availability
UCHT1 ; HCD14 ; 3G8 ; HIB19 ; 2H7 ; HCD56 ;
Regulatory Status
Other Names
Lin 1
Mouse IgG1, κ; Mouse IgG2b, κ
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Product Citations
Human peripheral blood leukocytes were stained with Pacific Blue™ anti-Human Lineage Cocktail and HLA-DR PerCP.
  • PB_Human_Lineage_cocktail_Antibody_FC_1_101912
    Human peripheral blood leukocytes were stained with Pacific Blue™ anti-Human Lineage Cocktail and HLA-DR PerCP.
  • PB_Human_Lineage_cocktail_Antibody_FC_2_101912
    Human peripheral blood leukocytes co-stained with CD123 PE and HLA-DR PerCP (gated on the Lineage negative/dim cell population).
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348805 50 tests £407.00
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This anti-Human Lineage Cocktail is optimized for the detection of human peripheral blood T cells, B cells, NK cells, monocytes, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells and/or basophils. This cocktail is composed of CD3, CD14, CD16, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD16 is expressed on NK cells, activated monocytes/macrophages, and neutrophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey. (FC)
Product Citations
  1. Hu EY, et al. 2020. JCI Insight. 5:00. PubMed
  2. Moravcikova E, et al. 2018. Cytometry A. 93:894. PubMed
AB_2889063 (BioLegend Cat. No. 348805)

Antigen Details


T cells, B cells, NK cells, monocytes/macrophages, neutrophils

Cell Type
B cells, Dendritic cells, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, NK cells, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Gene ID
915 View all products for this Gene ID 2214 View all products for this Gene ID 930 View all products for this Gene ID 931 View all products for this Gene ID 4684 View all products for this Gene ID 929 View all products for this Gene ID
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Go To Top Version: 2    Revision Date: 07/09/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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