NMDA (GluN) Receptor Antibody Sampler Kit

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NMDA-Receptor_Antibody_Sampler_Kit_1_110318
Western blot of purified anti-NMDAR1 antibody (clone N308/48). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NMDA-Receptor_Antibody_Sampler_Kit_1_110318
    Western blot of purified anti-NMDAR1 antibody (clone N308/48). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NMDA-Receptor_Antibody_Sampler_Kit_2_110318
    Western blot of purified anti-NMDAR2A antibody (clone N327/95). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NMDA-Receptor_Antibody_Sampler_Kit_3_110318
    Western blot of purified anti-NMDAR2B antibody (clone N59/36). Lane 1: Molecular weight marker; Lane 2: 30 µg of human brain membrane lysate; Lane 3: 5 µg of mouse brain membrane lysate; Lane 4: 5 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NMDA-Receptor_Antibody_Sampler_Kit_4_110318
    Western blot of purified anti-PSD95 antibody (clone K28/74). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain membrane lysate; Lane 3: 10 µg of mouse brain membrane lysate; Lane 4: 10 µg of rat brain membrane lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NMDA-Receptor_Antibody_Sampler_Kit_5_110318
    Western blot of purified anti-Pan-Shank antibody (clone N23B/49). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of N23B/49 or mouse IgG1, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • NMDA-Receptor_Antibody_Sampler_Kit_6_110318
    IHC staining of purified anti-Pan-Shank antibody (clone N23B/49) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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899908 1 kit £188
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Description

The NMDA (GluN) Receptor Antibody Sampler Kit offers flexibility for sampling and detection of NMDA receptor subunits GluN1, GluN2A and GluN2B. The kit also includes key postsynaptic targets, PSD95 and Shank, to allow protein localization assessment. These antibodies are highly suitable for WB and IHC applications.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Clone Size Reactivity Isotype
Anti-NMDAR1 N308/48 25 μl Rat, Mouse Mouse IgG1
Anti-NMDAR2A N327/95 25 μl Mouse, Rat Mouse IgG2a
Anti-NMDAR2B N59/36 25 μl Human, Mouse, Rat Mouse IgG2b
Anti-PSD95 K28/74 25 μl Rat, Mouse Mouse IgG1
Anti-Pan-Shank N23B/49 25 μg Human, Mouse, Rat Mouse IgG1, κ

* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Antibody Type
Monoclonal
Formulation
Please refer to individual product datasheets of the purified formats for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-NMDAR1: 2.0 - 10 µg/ml
Anti-NMDAR2A: 10 µg/ml
Anti-NMDAR2B: 1.0 - 5.0 µg/ml
Anti-PSD95: 5.0 - 10 µg/ml
Anti-Pan-Shank: 1.0 - 5.0 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For validated or reported applications for these antibodies, please see individual product datasheets.

Related FAQs

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Go To Top Version: 1    Revision Date: 11/02/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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