Mouse Regulatory B Cell (B10) Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647)

Pricing & Availability
Clone
6D5, 53-7.3, 1B1 (See other available formats)
Other Names
Breg, B regulatory
Isotype
Rat IgG2a, Rat IgG2b
Ave. Rating
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Product Citations
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A_B10FlowKit_050109.jpg
C57BL/6 mouse splenocytes were stained with Mouse Regulatory B Cell Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647). Histogram shows staining with CD19 FITC; dot plot data shows staining with CD1d Alexa Fluor® 647 and CD5 PE on gated CD19+ cells.
  • A_B10FlowKit_050109.jpg
    C57BL/6 mouse splenocytes were stained with Mouse Regulatory B Cell Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647). Histogram shows staining with CD19 FITC; dot plot data shows staining with CD1d Alexa Fluor® 647 and CD5 PE on gated CD19+ cells.
  • z6D5_53-7.3_1B1_061410
    C57BL/6 mouse splenocytes were stained with Mouse Regulatory B Cell Flow Kit (CD19 FITC/CD5 PE/CD1d Alexa Fluor® 647). Histogram shows staining with CD19 FITC; dot plot data shows staining with CD1d Alexa Fluor® 647 and CD5 PE on gated CD19+ cells.
Cat # Size Price Quantity Avail. Save
132601 25 tests £202
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Description

Splenic IL-10-producing CD1dhighCD5+ B cells have been designated as regulatory B cells (Breg or B10). These rare IL-10- producing B cells represent only 1%-2% of spleen B cells and 7%-8% of peritoneal B cells in mice but are not normally detectable in blood or lymph nodes. B10 cells are a unique subset of potent regulatory B cells different from B-1a, B-1b, or CD21+CD23- mantle zone B cells. B10 cells produce IL-10 and negatively regulate T cell function. BioLegend's Mouse Regulatory B Cell Flow Kit is composed of fluorochrome conjugated anti-mouse CD19, CD5, and CD1d. It is optimized for detection or isolation of B10 cells by flow cytometry.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
This kit is guaranteed for nine months. Upon receipt, store between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product.

This product has a shelf-life of 12 months or less. Please contact our technical support team for lot specific CoA and expiration date inquiries of this product.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells.

Materials provided:
FITC anti-mouse CD19/PE anti-mouse CD5/Alexa Fluor® 647 anti-mouse CD1d, 25 tests
FITC Rat IgG2a/PE Rat IgG2a/Alexa Fluor® 647 Rat IgG2b isotype controls, 25 tests

Materials not provided:
Cell Staining Buffer (Cat. No. 420201)
TruStain FcX (Cat. No. 101319)
Single color compensation controls

Antigen Details

Distribution

Represent 1%-2% of spleen B cells in wild-type mice, but expand to about 10% of spleen B cells in hCD19Tg mice.

Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

Yanaba K, et al. 2008. Immunity 25:639.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/03/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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